Background and Objectives: Trypsin urine (UTI) inhibitors, protein inhibiting diverse synthesized with hepatocytes, are considered to play an important role not only in organ injury protection during severe inflammation but also in the inhibition of tumor invasion and metastasis. However, the precision mechanism that underlies its synthesis control, secretion, and processing is still not recognized. The purpose of this study is to determine whether human hepatoma cells hepatoma issue an ISK in free form and whether the synthesis and secretion are regulated by the proinflammatory cytokine. Method: HEPG2 cells that are cultivated by distimulating using different interleukin-6 concentrations (IL-6), factors of tumor-alpha (TNF-Alpha) necrosis and interleukin-1beta (IL-1beta).
The free concentration of UTI in the media was measured by ELISA and intracellular ISK precursors identified by West Blotting. Iti MRNA expressions are studied with the reverse transcription polymerase chain reaction (RT-PCR). Results: HEPG2 cells constantly releasing free UTIs and these secretions are significantly regulated by IL-6, IL-1beta, and TNF-Alpha. IL-6, IL-1Beta, and TNF-Alpha enhances the synthesis of intracellular ISUK precursor proteins, and IL-1beta MRNA expressions are set. Conclusion: HEPG2 cells constantly release free uti. Proinflammatory cytokines, IL-1beta, IL-6, and TNF-alpha, set the synthesis and secretion of UTI by regulating MRNA UTI expressions.
The uroepithelial cells of the human bladder synergize with a monocyte to promote IL-10 synthesis and other cytokine responses towards Uropatogenic Escherichia coli.
Urinary tract infections are the main source of morbidity for women and the elderly, with Uropatogenic Escherichia coli (UPEC) to be the most common cause pathogen. Studies in recent years have defined the main anti-inflammatory role for interleukin-10 (IL-10) in urinary tract infections mediated by UPEC and other uropathogens. We investigate the nature of the interaction that produces IL-10 between UPEC and host cells by utilizing a new culture model that includes lymphocytes, mononuclear cells and uroepithelials in the proportion of histootypes. This culture model shows the effects of the production of IL-10 synergist between monocytes and uroepithelial cells after the infection with the UPEC.
Membrane inserts are used to separate the type of monocyte cell and uroepitel during infection and reveal two synergistic IL-10 production effects based on interaction that depends on contact and dissolves. Analysis of a comprehensive set of biomarkers relevant immunized in monocyte-uroepithelial cell culture highlights that some cytokines, kemokines and signal factors are also produced synergistically or antagonists. These results indicate that the IL-10 response to the UPEC occurred through several interactions between several cells, implying a complex role for IL-10 related to infection during the ISK. The development and implementation of the co-cultural model described in this study is thus useful to determine the level of contact with the Biomarker production contacts to UPEC, and highlight the relevance of the Histotype Cooperative in studying complex host-pathogenic interactions.
The theory of cognition based assumes that conceptual representation is based on sensorimotor experience. However, abstract concepts such as jealousy or childhood do not have direct-related references with such sensorimotor experience can be done; Therefore, the foundation of the abstract concept has long been the topic of debate. Here, we propose (a) that systematic relationships exist between semantic representations learned from languages on one side and perception experience on the other side, (b) that this relationship can be studied in bottom-up mode, and (c) it is possible to extrapolate From this learning experience to predict the representation of the perception expected for words even where the experience is directly lost.
To test this, we apply computing models driven by data trained to map language-based representations (obtained from the Corpora text, representing a language experience) to a vision-based representation (obtained from the image database, which represents perceptual experience), and applies The mapping function is to a language-based representation for abstract and concrete words outside the training set. In three experiments, we present participants with these words, accompanied by two images: images predicted by random control models and images.
The results showed that the assessment of participants was in line with the model prediction even for the most abstract words. This preference is stronger for more concrete items and decreases for more abstract. Simultaneously, our findings have substantial implications in supporting abstract words, showing that we can take advantage of our previous experience to create possible visual representations that we do not have.
In total hip arthroplasties, using particles produced in surface articulation and interfaces between bones, cement and implants have a negative impact on osteoblasts, which cause osteolysis and implants loosen. The purpose of this experimental study is to determine the effects of particulatory wear debris produced on the interface between straight stainless steel hip stems (Exeter (®)) and three different bone cement (Palacos (®) R, Simplex ™ P and Cemex (® ) Genta) on cell viability, collagen synthesis and cytokine expression on human osteoblasts. Primary osteoblasts are treated with various concentrations of particles using. Procollagen type synthesis I and different cytokines are analyzed, and markers for apoptosis and necrosis are also detected.
The level of cytokine synthesis in osteoblasts initially increased and varied, depending on the incubation time and the concentration of particles. Specific differences in the synthesis level of interleukin (IL) -6, IL-8, the growth factor of vascular endotheleal (VEGF) and Chemotactic monocyte-1 protein (MCP-1) are observed with various cement bones studied. The negative effects of particles at procollagen type I synthesis and increased levels of cell apoptosis and necrosis were observed with the three cement analyzed.
Human Interleukin-10 |
90173-A |
BPS Bioscience |
2 µg |
EUR 130 |
Description: Recombinant Human Interleukin-10 is a disulfide-linked monomer protein consisting of 161 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-10 mature chain was expressed in E. coli. |
Human Interleukin-10 |
90173-B |
BPS Bioscience |
10 µg |
EUR 205 |
Description: Recombinant Human Interleukin-10 is a disulfide-linked monomer protein consisting of 161 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-10 mature chain was expressed in E. coli. |
Mouse Interleukin-10 |
90174-A |
BPS Bioscience |
2 µg |
EUR 130 |
Description: Recombinant Mouse IL-10 is a disulfide-linked monomer protein consisting of 161 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding mouse Interleukin-10 mature chain was expressed in E. coli. |
Mouse Interleukin-10 |
90174-B |
BPS Bioscience |
10 µg |
EUR 205 |
Description: Recombinant Mouse IL-10 is a disulfide-linked monomer protein consisting of 161 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding mouse Interleukin-10 mature chain was expressed in E. coli. |
Interleukin-10 (IL-10) Rat |
GWB-51A291 |
GenWay Biotech |
0.01 mg |
Ask for price |
|
IL-10, Interleukin-10, rat |
RC252-21 |
Bio Basic |
2ug |
EUR 125.26 |
|
Rat Interleukin 10 |
90175-A |
BPS Bioscience |
5 µg |
EUR 130 |
Description: Recombinant Rat Interleukin-10 is a disulfide-linked monomer protein consisting of 161 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding rat Interleukin-10 mature chain was expressed in E. coli. |
Rat Interleukin 10 |
90175-B |
BPS Bioscience |
20 µg |
EUR 205 |
Description: Recombinant Rat Interleukin-10 is a disulfide-linked monomer protein consisting of 161 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding rat Interleukin-10 mature chain was expressed in E. coli. |
Interleukin-10 Antibody |
GWB-38184B |
GenWay Biotech |
0.5 mg |
Ask for price |
Interleukin-10 Antibody |
GWB-DB0169 |
GenWay Biotech |
0.1 mg |
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Interleukin-10 Antibody |
GWB-8AC374 |
GenWay Biotech |
0.5 mg |
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Interleukin-10 Antibody |
GWB-8FC0AE |
GenWay Biotech |
0.5 mg |
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Interleukin-10 (IL-10) Human |
GWB-849240 |
GenWay Biotech |
0.01 mg |
Ask for price |
Interleukin-10 (IL-10) Mouse |
GWB-92B4DF |
GenWay Biotech |
0.01 mg |
Ask for price |
IL-10, Interleukin-10, human |
RC212-21 |
Bio Basic |
2ug |
EUR 125.26 |
|
IL-10/Interleukin-10, Antibody |
GWB-9B391B |
GenWay Biotech |
1 ml |
Ask for price |
INTERLEUKIN 10, Antibody |
GWB-31B7C8 |
GenWay Biotech |
0.1 mg |
Ask for price |
|
INTERLEUKIN 10, Antibody |
GWB-BB3DD6 |
GenWay Biotech |
0.5 mg |
Ask for price |
Interleukin-10 ELISA Kit |
GWB-3D36C0 |
GenWay Biotech |
96 TESTS |
Ask for price |
|
Sheep Interleukin-10 (IL10) |
1-CSB-YP640904SH |
Cusabio |
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|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Sheep Interleukin-10(IL10) expressed in Yeast |
Human Interleukin-10 (IL10) |
1-CSB-RP065944h |
Cusabio |
-
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|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Interleukin-10(IL10) expressed in E.coli |
Bovine Interleukin-10 (IL10) |
1-CSB-YP011580BO |
Cusabio |
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- 10ug
- 1MG
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- 500ug
- 50ug
|
|
Description: Recombinant Bovine Interleukin-10(IL10) expressed in Yeast |
Rat Interleukin-10 Antibody |
GWB-3EA6D5 |
GenWay Biotech |
0.1 mg |
Ask for price |
Interleukin-10 Antibody (RPE) |
GWB-D6BF53 |
GenWay Biotech |
100 TESTS |
Ask for price |
Interleukin 10 (IL-10) Antibody |
abx432852-200ul |
Abbexa |
200 ul |
EUR 343.2 |
|
Interleukin 10 (IL-10) Antibody |
abx234211-100ug |
Abbexa |
100 ug |
EUR 661.2 |
|
Interleukin 10 (IL-10) Antibody |
abx234212-100ug |
Abbexa |
100 ug |
EUR 661.2 |
|
Interleukin 10 (IL-10) Antibody |
abx234211-100g |
Abbexa |
100 µg |
EUR 350 |
Interleukin 10 (IL-10) Antibody |
abx234212-100g |
Abbexa |
100 µg |
EUR 350 |
Interleukin 10 (IL-10) Antibody |
abx432852-100g |
Abbexa |
100 µg |
EUR 250 |
Mouse Interleukin-10 Antibody |
GWB-AA8138 |
GenWay Biotech |
0.1 mg |
Ask for price |
Mouse Interleukin-10 Antibody |
GWB-816E6C |
GenWay Biotech |
0.5 mg |
Ask for price |
IL-10, Interleukin-10, murine (mouse) |
RC232-21 |
Bio Basic |
2ug |
EUR 125.26 |
|
Recombinant Rat Interleukin-10 |
CC104-002 |
GenDepot |
2ug |
EUR 351.6 |
Recombinant Rat Interleukin-10 |
CC104-010 |
GenDepot |
10ug |
EUR 489.6 |
Recombinant Rat Interleukin-10 |
CC104-101 |
GenDepot |
1mg |
EUR 12372 |
Recombinant Rat Interleukin-10 |
7-00922 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Rat Interleukin-10 |
7-00923 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Rat Interleukin-10 |
7-00924 |
CHI Scientific |
1mg |
Ask for price |
Recombinant Rat Interleukin-10 |
AP60234 |
SAB |
100ug |
EUR 1554 |
|
Interleukin-10 Antibody (Biotin) |
GWB-B59216 |
GenWay Biotech |
0.5 mg |
Ask for price |
Bovine Interleukin-10 Antibody |
GWB-Q00845 |
GenWay Biotech |
0.5 mg |
Ask for price |
Interleukin-10 Antibody (Biotin) |
GWB-90CD5F |
GenWay Biotech |
0.2 mg |
Ask for price |
Interleukin-10 Antibody (Biotin) |
GWB-F14B90 |
GenWay Biotech |
0.05 mg |
Ask for price |
Recombinant Rat Interleukin-10 |
cyt-465-10g |
ProSpec Tany |
10µg |
EUR 145 |
Recombinant Rat Interleukin-10 |
cyt-465-1mg |
ProSpec Tany |
1mg |
EUR 4680 |
Recombinant Rat Interleukin-10 |
cyt-465-2g |
ProSpec Tany |
2µg |
EUR 60 |
Interleukin-10 Rat Recombinant |
rAP-0589 |
Angio Proteomie |
Inquiry |
Ask for price |
Anti- Interleukin-10 (IL-10) Antibody |
GWB-2BD01D |
GenWay Biotech |
0.05 mg |
Ask for price |
|
Active Interleukin 10 (IL10) |
APA056Bo61 |
Cloud-Clone |
10ug |
EUR 368 |
Active Interleukin 10 (IL10) |
APA056Ca61 |
Cloud-Clone |
10ug |
EUR 328 |
Active Interleukin 10 (IL10) |
APA056Eq61 |
Cloud-Clone |
10ug |
EUR 380 |
Active Interleukin 10 (IL10) |
APA056Ga61 |
Cloud-Clone |
10ug |
EUR 319 |
Active Interleukin 10 (IL10) |
APA056Hu61 |
Cloud-Clone |
10ug |
EUR 335 |
Active Interleukin 10 (IL10) |
4-APA056Hu61 |
Cloud-Clone |
-
Ask for price
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|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Interleukin 10 expressed in: 293F cell |
Active Interleukin 10 (IL10) |
APA056Mu61 |
Cloud-Clone |
10ug |
EUR 324 |
Active Interleukin 10 (IL10) |
APA056Ra01 |
Cloud-Clone |
10ug |
EUR 350 |
Active Interleukin 10 (IL10) |
APA056Ra61 |
Cloud-Clone |
10ug |
EUR 251 |
Active Interleukin 10 (IL10) |
APA056Rb61 |
Cloud-Clone |
10ug |
EUR 331 |
Human Interleukin-10 (IL-10) Antibody |
13109-05011 |
AssayPro |
150 ug |
EUR 175 |
IL-10/Interleukin-10 (mouse), Antibody |
GWB-C42D3C |
GenWay Biotech |
1 ml |
Ask for price |
Rat Interleukin-10 ELISA Kit |
EK1149 |
SAB |
96 tests |
EUR 511 |
|
Recombinant Human Interleukin-10 |
CC054-002 |
GenDepot |
2ug |
EUR 351.6 |
Recombinant Human Interleukin-10 |
CC054-010 |
GenDepot |
10ug |
EUR 489.6 |
Recombinant Human Interleukin-10 |
CC054-101 |
GenDepot |
1mg |
EUR 10752 |
Recombinant Mouse Interleukin-10 |
CC075-002 |
GenDepot |
2ug |
EUR 351.6 |
Recombinant Mouse Interleukin-10 |
CC075-010 |
GenDepot |
10ug |
EUR 489.6 |
Recombinant Mouse Interleukin-10 |
CC075-101 |
GenDepot |
1mg |
EUR 9618 |
Recombinant Human Interleukin-10 |
7-00913 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Interleukin-10 |
7-00914 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Interleukin-10 |
7-00915 |
CHI Scientific |
1mg |
Ask for price |
Recombinant Mouse Interleukin-10 |
7-00919 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Mouse Interleukin-10 |
7-00920 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Mouse Interleukin-10 |
7-00921 |
CHI Scientific |
1mg |
Ask for price |
Recombinant Mouse Interleukin-10 |
cyt-497-10g |
ProSpec Tany |
10µg |
EUR 145 |
Recombinant Mouse Interleukin-10 |
cyt-497-1mg |
ProSpec Tany |
1mg |
EUR 4680 |
Recombinant Mouse Interleukin-10 |
cyt-497-2g |
ProSpec Tany |
2µg |
EUR 60 |
Recombinant Human Interleukin-10 |
cyt-500-10g |
ProSpec Tany |
10µg |
EUR 145 |
Recombinant Human Interleukin-10 |
cyt-500-1mg |
ProSpec Tany |
1mg |
EUR 4680 |
Recombinant Human Interleukin-10 |
cyt-500-2g |
ProSpec Tany |
2µg |
EUR 60 |
Interleukin-10 Mouse Recombinant |
rAP-0603 |
Angio Proteomie |
Inquiry |
Ask for price |
Interleukin-10 Human Recombinant |
rAP-0604 |
Angio Proteomie |
Inquiry |
Ask for price |
Rat IL-10(Interleukin-10) ELISA Kit |
ER0033 |
FN Test |
96T |
EUR 571.5 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml |
Rat IL-10(Interleukin-10) ELISA Kit |
E39ER0033 |
EnoGene |
96T |
EUR 595 |
Rat Interleukin-10 (IL-10) ELISA Kit |
IRTIL10KT |
Innovative research |
each |
EUR 702 |
|
Description: Rat Interleukin-10 (IL-10) ELISA Kit |
Recombinant Murine Interleukin-10 |
AP60158 |
SAB |
100ug |
EUR 1554 |
|
Interleukin 10 (IL10) Antibody |
20-abx323722 |
Abbexa |
-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx101173 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx101174 |
Abbexa |
-
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-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx101175 |
Abbexa |
-
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-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx101176 |
Abbexa |
-
Ask for price
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx130143 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx130919 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx130920 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
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|
|
Interleukin 10 (IL10) Antibody |
20-abx130921 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx131817 |
Abbexa |
-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx131818 |
Abbexa |
-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx131819 |
Abbexa |
-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx131820 |
Abbexa |
-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx132171 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx173049 |
Abbexa |
-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx173050 |
Abbexa |
-
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-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx173051 |
Abbexa |
-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx173052 |
Abbexa |
-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx173053 |
Abbexa |
-
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-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx173054 |
Abbexa |
-
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-
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-
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-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx173055 |
Abbexa |
-
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|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx173056 |
Abbexa |
-
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-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx173057 |
Abbexa |
-
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-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx177073 |
Abbexa |
-
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-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx177074 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx177075 |
Abbexa |
-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx177076 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx177077 |
Abbexa |
-
Ask for price
-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx177078 |
Abbexa |
-
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-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx137087 |
Abbexa |
-
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-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx137098 |
Abbexa |
-
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-
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|
|
|
Interleukin 10 (IL10) Antibody |
20-abx109331 |
Abbexa |
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx109411 |
Abbexa |
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx104900 |
Abbexa |
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- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Interleukin 10 (IL10) Antibody |
20-abx104934 |
Abbexa |
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- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
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The proinflammatory cytokine turns on the synthesis and secretion of urine trypsin inhibitors in human hepatoma cells.
Our current data shows that using particles from the interface between total hip stems and cement bone has a significant influence on viability, cytokine expression, and collagen synthesis in human osteoblasts, depending on bone cement.