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Learn at the synthesis of complement components by human kidney mesangial cells in culture. Assessment of cytokine effects.

Learn at the synthesis of complement components by human kidney mesangial cells in culture. Assessment of cytokine effects.
Posted by Frank

Cultivation of kidney mesangial cells (MC) is easily established and widely used. MCs produce several complementary regulatory proteins (C). We studied whether MC synthesized components C (C3, C5, C8). MC culture was established from the normal portion of nephrectomy for kidney cancer. After growing to a close meeting in RPMI / 17% FBS and resting 24 hours in RPMI / 0.5% FBS, MC stimulated up to 72 hours with IL-1β or IL-6 (10, 100, 1000 U / mL).

Both C5 and C8 detection by ELISA. While C3 is present in supernatant in basal conditions (15.5-107.6 ng / 10 (6) cells / 24 hours) in different MC lines. IL-1β regulates synthesis with 2.4-4.5 folds, while IL-6 does not show any effect. The c3 synthetic rate is cell 1.76 ng / 10 (6) under IL-1 stimulation versus basal level 0.37 ng / hour / 10 (6) cells. MC C3 production, especially induced by IL-1 may have pathogenetic relevance in glomerulonephritis.

Rooperol, cytokine synthesis inhibitors, reduces breathing explosions on leukocytes and human macrophies and mice.

Chemiluminescence enhanced by Luminol is measured in all fresh human blood, or human neutrophils are isolated from the blood of heparation, macrophages of alveolar humans and macrophages of alveolar rats which are stimulated with endotoxin bacteria (LPS). Esther TetraAceTate from Rooperol, a recatechol that shows anticoytokine activity, added to the cell simultaneously with LPS inhibiting the breathing explosion. The effective concentration of Rooperol is in the range of 1-10 mothers depending on the type of cell and in accordance with the inhibition of the production of nitrate oxide by macrophages of mice alveolar. Thus Roerol can reduce some effects of excessive phagocytic activity and inflammatory reactions but by extinguishing free radical production can also reduce resistance to bacterial infections.

Leptin increases survival and induces migration, degranulation, and synthesis of cytokines from human basophyl.

Basophils are the rarest leukocytes in human blood, but are now recognized as one of the most important immunomodulatory cells and the effector in allergic inflammation. Leptin, a member of the Cytokine IL-6 family, has a metabolic effect as an adipocine, and is also known to participate in the pathogenesis of inflammatory reactions. Because there is an epidemiological relationship between obesity and allergies, we examine whether the basophyl function is modified by leptin.

We found that the human basophyl expressed Leptin receptor (LEPR) at MRNA and surface protein levels, which was regulated by IL-33. Leptin exerts strong effects on several basophyl functions. This causes a strong migration response to human basophyl, potentially similar to the basophil-active kemokine. Also, Leptin increases the survival of human basophyl, even though its potential is less than IL-3. In addition, CD63, the marker of basophil activation is stated on the cell surface, regulated by leptin, the effect is neutralized by blocking LEPR.

Basophyl degranulation assessment and cytokine synthesis found that leptin shows a strong priming effect on human basophyl degranulation in response to FCεRI aggregation and induces Th2, but not the production of cytokines by cells. In short, these findings indicate that leptin may be the main molecule that mediates the effects of adipocytes in inflammatory cells such as basophiles by binding LEPR and activating cellular functions, which may worsen allergic inflammation.

Learn at the synthesis of complement components by human kidney mesangial cells in culture. Assessment of cytokine effects.

Cultivation of kidney mesangial cells (MC) is easily established and widely used. MCs produce several complementary regulatory proteins (C). We studied whether MC synthesized components C (C3, C5, C8). MC culture was established from the normal portion of nephrectomy for kidney cancer. After growing to a close meeting in RPMI / 17% FBS and resting 24 hours in RPMI / 0.5% FBS, MC stimulated up to 72 hours with IL-1β or IL-6 (10, 100, 1000 U / mL). Both C5 and C8 detection by ELISA.

While C3 is present in supernatant in basal conditions (15.5-107.6 ng / 10 (6) cells / 24 hours) in different MC lines. IL-1β regulates synthesis with 2.4-4.5 folds, while IL-6 does not show any effect. The c3 synthetic rate is cell 1.76 ng / 10 (6) under IL-1 stimulation versus basal level 0.37 ng / hour / 10 (6) cells. MC C3 production, especially induced by IL-1 may have pathogenetic relevance in glomerulonephritis.

Rooperol, cytokine synthesis inhibitors, reduces breathing explosions on leukocytes and human macrophies and mice.

Chemiluminescence enhanced by Luminol is measured in all fresh human blood, or human neutrophils are isolated from the blood of heparation, macrophages of alveolar humans and macrophages of alveolar rats which are stimulated with endotoxin bacteria (LPS). Esther TetraAceTate from Rooperol, a recatechol that shows anticoytokine activity, added to the cell simultaneously with LPS inhibiting the breathing explosion. The effective concentration of Rooperol is in the range of 1-10 mothers depending on the type of cell and in accordance with the inhibition of the production of nitrate oxide by macrophages of mice alveolar. Thus Roerol can reduce some effects of excessive phagocytic activity and inflammatory reactions but by extinguishing free radical production can also reduce resistance to bacterial infections.

Learn at the synthesis of complement components by human kidney mesangial cells in culture. Assessment of cytokine effects.

Leptin increases survival and induces migration, degranulation, and synthesis of cytokines from human basophyl.

Basophils are the rarest leukocytes in human blood, but are now recognized as one of the most important immunomodulatory cells and the effector in allergic inflammation. Leptin, a member of the Cytokine IL-6 family, has a metabolic effect as an adipocine, and is also known to participate in the pathogenesis of inflammatory reactions. Because there is an epidemiological relationship between obesity and allergies, we examine whether the basophyl function is modified by leptin. We found that the human basophyl expressed Leptin receptor (LEPR) at MRNA and surface protein levels, which was regulated by IL-33.

Leptin exerts strong effects on several basophyl functions. This causes a strong migration response to human basophyl, potentially similar to the basophil-active kemokine. Also, Leptin increases the survival of human basophyl, even though its potential is less than IL-3. In addition, CD63, the marker of basophil activation is stated on the cell surface, regulated by leptin, the effect is neutralized by blocking LEPR. Basophyl degranulation assessment and cytokine synthesis found that leptin shows a strong priming effect on human basophyl degranulation in response to FCεRI aggregation and induces Th2, but not the production of cytokines by cells.

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Recombinant Human CD2

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Recombinant Human CD2

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Recombinant Human CD9

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Recombinant Human CD9

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Recombinant Human p53

7-05785 2µg Ask for price

Recombinant Human p53

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Recombinant Human PRL

CM10-10ug 10ug
EUR 169.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human PRL

CM10-1mg 1mg
EUR 2008.8
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human PRL

CM10-500ug 500ug
EUR 1423.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant Human PRL

CM10-50ug 50ug
EUR 363.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.

Recombinant human EDA

P1422 100ug Ask for price
Description: Recombinant protein for human EDA

Recombinant human ENG

P1433 100ug Ask for price
Description: Recombinant protein for human ENG

Recombinant human PXN

P1439 100ug Ask for price
Description: Recombinant protein for human PXN

Recombinant human FAS

P1464 100ug Ask for price
Description: Recombinant protein for human FAS

Recombinant human REL

P1479 100ug Ask for price
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Recombinant human MET

P1995 100ug Ask for price
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Recombinant human KIT

P1996 100ug Ask for price
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Recombinant human TEC

P1997 100ug Ask for price
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Recombinant human FRY

P2014 100ug Ask for price
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Recombinant human VHL

P2120 100ug Ask for price
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Recombinant human FEV

P2435 100ug Ask for price
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Recombinant human GGN

P2440 100ug Ask for price
Description: Recombinant protein for human GGN

Recombinant human KLB

P2441 100ug Ask for price
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Recombinant human DBT

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Recombinant human MT4

P2447 100ug Ask for price
Description: Recombinant protein for human MT4

Recombinant Human CRP

P0007 100ug
EUR 626.83
Description: Recombinant Human protein for CRP

Recombinant Human PCT

P0010 100ug
EUR 626.83
Description: Recombinant Human protein for PCT

Recombinant Human AND

P0015 100ug
EUR 626.83
Description: Recombinant Human protein for AND

Recombinant Human CKB

P0020-B 100ug
EUR 626.83
Description: Recombinant Human protein for CKB

Recombinant Human CKM

P0020-M 100ug
EUR 626.83
Description: Recombinant Human protein for CKM

Recombinant Human EPO

P0026 100ug
EUR 626.83
Description: Recombinant Human protein for EPO

Recombinant Human HE4

P0034 100ug
EUR 626.83
Description: Recombinant Human protein for HE4

Recombinant Human NSE

P0039 100ug
EUR 626.83
Description: Recombinant Human protein for NSE

Recombinant Human NNE

P0040 100ug
EUR 626.83
Description: Recombinant Human protein for NNE

Recombinant Human PSA

P0041 100ug
EUR 626.83
Description: Recombinant Human protein for PSA

Recombinant Human HE4

P0059 100ug
EUR 626.83
Description: Recombinant Human protein for HE4

Recombinant Human YY1

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EUR 626.83
Description: Recombinant Human protein for YY1

Recombinant Human SAA

P0209 100ug
EUR 626.83
Description: Recombinant Human protein for SAA

Recombinant Human JNK

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EUR 626.83
Description: Recombinant Human protein for JNK

Recombinant Human ECS

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EUR 626.83
Description: Recombinant Human protein for ECS

Recombinant Human TBP

P0268 100ug
EUR 626.83
Description: Recombinant Human protein for TBP

Recombinant Human NPY

P0282 100ug
EUR 626.83
Description: Recombinant Human protein for NPY

Recombinant Human FYN

P0305 100ug
EUR 626.83
Description: Recombinant Human protein for FYN

Recombinant Human P65

P0336 100ug
EUR 626.83
Description: Recombinant Human protein for P65

Recombinant Human CBP

P0344 100ug
EUR 626.83
Description: Recombinant Human protein for CBP

Recombinant Human NIK

P0349 100ug
EUR 626.83
Description: Recombinant Human protein for NIK

Recombinant Human ADO

P0377 100ug
EUR 626.83
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Recombinant Human HK3

P0394 100ug
EUR 626.83
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Recombinant human FAN

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Description: Recombinant protein for human FAN

Recombinant human BI1

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Description: Recombinant protein for human BI1

Recombinant Human GLS

P0450 100ug
EUR 626.83
Description: Recombinant Human protein for GLS

Recombinant Human SRC

P0454 100ug
EUR 626.83
Description: Recombinant Human protein for SRC

Recombinant Human TAU

P0455 100ug
EUR 626.83
Description: Recombinant Human protein for TAU

Recombinant Human ME1

P0470 100ug
EUR 626.83
Description: Recombinant Human protein for ME1

Recombinant Human ADP

P0472 100ug
EUR 626.83
Description: Recombinant Human protein for ADP

Recombinant Human B2M

P0482 100ug
EUR 626.83
Description: Recombinant Human protein for B2M

Recombinant Human GLA

P0494 100ug
EUR 626.83
Description: Recombinant Human protein for GLA

Recombinant Human CAT

P0501 100ug
EUR 626.83
Description: Recombinant Human protein for CAT

Recombinant Human AFM

P0508 100ug
EUR 626.83
Description: Recombinant Human protein for AFM

Recombinant Human CA9

P0512 100ug
EUR 626.83
Description: Recombinant Human protein for CA9

Recombinant Human BAX

P0544 100ug
EUR 626.83
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Recombinant Human DAT

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Description: Recombinant protein for human SCX

Recombinant human SON

P1072 100ug Ask for price
Description: Recombinant protein for human SON

Recombinant Human TNF-?

SJA03-03 25µg/vial
EUR 282

Recombinant Human TNF-?

SJA03-04 50µg/vial
EUR 342

Recombinant Human SCF

SJA06-01 10µg/vial
EUR 342

Recombinant Human SCF

SJA06-02 100µg/vial
EUR 846

Recombinant Human EGF

SJA08-01 100µg/vial
EUR 168

Recombinant Human IFN-?

SJC03-01 20µg/vial
EUR 228

Recombinant Human IFN-?

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EUR 342

Recombinant Human CD7

RP-924 2 ug
EUR 416.4

Recombinant Human NSE

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HEENP-1601 100ug Ask for price

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HEGFP-0501 100ug Ask for price

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Recombinant Human EGF

EPT004 10ug
EUR 23.4
Description: Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed.

Recombinant Human PTH

EPT146 50ug
EUR 353.4
Description: Recombinant Human Parathyroid Hormone is produced by our E.coli expression system and the target gene encoding Ser32-Gln115 is expressed.

Recombinant Human LIF

EPT153 10ug
EUR 119.4
Description: Recombinant Human Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser23-Phe202 is expressed.

Recombinant Human C3a

EPT178 50ug
EUR 353.4
Description: Recombinant Human Complement Component C3a is produced by our E.coli expression system and the target gene encoding Ser672-Arg748 is expressed.

Recombinant Human SCF

EPT201 50ug
EUR 209.4
Description: Recombinant Human Stem Cell Factor is produced by our E.coli expression system and the target gene encoding Glu26-Ala189 is expressed.

Recombinant Human ACE2

ACE2-68H-20ug 20ug
EUR 684
Description: Signal peptide sequence and the extracellular domain of human ACE2 (aa 1-740) are fused at the C-terminus to the Fc portion of human IgG1

Recombinant Human ACE2

ACE2-68H-50ug 50ug
EUR 996
Description: Signal peptide sequence and the extracellular domain of human ACE2 (aa 1-740) are fused at the C-terminus to the Fc portion of human IgG2

Recombinant Human ACE2

ACE2-736H-20ug 20ug
EUR 684
Description: Recombinant Human Angiotensin-Converting Enzyme 2/ACE-2 produced by transfected human cells is a secreted protein with sequence (Gln18-Ser740) of human ACE-2 fused with a polyhistidine tag at the C-terminus.The encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

Recombinant Human ACE3

ACE2-736H-50ug 50ug
EUR 996
Description: Recombinant Human Angiotensin-Converting Enzyme 2/ACE-2 produced by transfected human cells is a secreted protein with sequence (Gln18-Ser740) of human ACE-2 fused with a polyhistidine tag at the C-terminus.The encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

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Recombinant Human CD23

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Recombinant Human CD23

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7-06048 1mg Ask for price

Recombinant Human SOX9

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7-06486 10µg Ask for price

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Description: Recombinant protein for human FLCN

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Description: Recombinant protein for human FNTA

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Description: Recombinant protein for human GLG1

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Description: Recombinant protein for human DNM2

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Description: Recombinant protein for human CTCF

Recombinant human CIKS

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Description: Recombinant protein for human CD63

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P1933 100ug Ask for price
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Description: Recombinant protein for human FRYL

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P1994 100ug Ask for price
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P2002 100ug Ask for price
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Recombinant human PLD1

P2005 100ug Ask for price
Description: Recombinant protein for human PLD1

Recombinant human TLR4

P2006 100ug Ask for price
Description: Recombinant protein for human TLR4

Recombinant human RTTN

P2009 100ug Ask for price
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Recombinant human OTOG

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Recombinant human CPTP

P2013 100ug Ask for price
Description: Recombinant protein for human CPTP

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P2016 100ug Ask for price
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P2017 100ug Ask for price
Description: Recombinant protein for human PIRT

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P2018 100ug Ask for price
Description: Recombinant protein for human RYR3

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P2021 100ug Ask for price
Description: Recombinant protein for human 2B14

Recombinant human FICD

P2022 100ug Ask for price
Description: Recombinant protein for human FICD

Recombinant human HIC1

P2026 100ug Ask for price
Description: Recombinant protein for human HIC1

Recombinant human K22O

P2030 100ug Ask for price
Description: Recombinant protein for human K22O

Recombinant human MIB2

P2037 100ug Ask for price
Description: Recombinant protein for human MIB2

Recombinant human MYH2

P2039 100ug Ask for price
Description: Recombinant protein for human MYH2

Recombinant human ALBU

P2041 100ug Ask for price
Description: Recombinant protein for human ALBU

Recombinant human CCL1

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Description: Recombinant protein for human CCL1

Recombinant human CCR2

P2048 100ug Ask for price
Description: Recombinant protein for human CCR2

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Description: Recombinant protein for human CCR5

Recombinant human CCR7

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Recombinant human CCR8

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Description: Recombinant protein for human CCR8

Recombinant human TAF4

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Recombinant human TLR5

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Description: Recombinant protein for human TLR5

Recombinant human MERL

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Description: Recombinant protein for human MERL

Recombinant human K2C1

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Description: Recombinant protein for human K2C1

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Description: Recombinant protein for human HXB3

Recombinant human ISK9

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Description: Recombinant protein for human ISK9

Recombinant human TBB5

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Recombinant human FGF8

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Description: Recombinant protein for human FGF8

Recombinant human PPB1

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Description: Recombinant protein for human PPB1

In short, these findings indicate that leptin may be the main molecule that mediates the effects of adipocytes in inflammatory cells such as basophiles by binding LEPR and activating cellular functions, which may worsen allergic inflammation.

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