Cultivation of kidney mesangial cells (MC) is easily established and widely used. MCs produce several complementary regulatory proteins (C). We studied whether MC synthesized components C (C3, C5, C8). MC culture was established from the normal portion of nephrectomy for kidney cancer. After growing to a close meeting in RPMI / 17% FBS and resting 24 hours in RPMI / 0.5% FBS, MC stimulated up to 72 hours with IL-1β or IL-6 (10, 100, 1000 U / mL).
Both C5 and C8 detection by ELISA. While C3 is present in supernatant in basal conditions (15.5-107.6 ng / 10 (6) cells / 24 hours) in different MC lines. IL-1β regulates synthesis with 2.4-4.5 folds, while IL-6 does not show any effect. The c3 synthetic rate is cell 1.76 ng / 10 (6) under IL-1 stimulation versus basal level 0.37 ng / hour / 10 (6) cells. MC C3 production, especially induced by IL-1 may have pathogenetic relevance in glomerulonephritis.
Rooperol, cytokine synthesis inhibitors, reduces breathing explosions on leukocytes and human macrophies and mice.
Chemiluminescence enhanced by Luminol is measured in all fresh human blood, or human neutrophils are isolated from the blood of heparation, macrophages of alveolar humans and macrophages of alveolar rats which are stimulated with endotoxin bacteria (LPS). Esther TetraAceTate from Rooperol, a recatechol that shows anticoytokine activity, added to the cell simultaneously with LPS inhibiting the breathing explosion. The effective concentration of Rooperol is in the range of 1-10 mothers depending on the type of cell and in accordance with the inhibition of the production of nitrate oxide by macrophages of mice alveolar. Thus Roerol can reduce some effects of excessive phagocytic activity and inflammatory reactions but by extinguishing free radical production can also reduce resistance to bacterial infections.
Leptin increases survival and induces migration, degranulation, and synthesis of cytokines from human basophyl.
Basophils are the rarest leukocytes in human blood, but are now recognized as one of the most important immunomodulatory cells and the effector in allergic inflammation. Leptin, a member of the Cytokine IL-6 family, has a metabolic effect as an adipocine, and is also known to participate in the pathogenesis of inflammatory reactions. Because there is an epidemiological relationship between obesity and allergies, we examine whether the basophyl function is modified by leptin.
We found that the human basophyl expressed Leptin receptor (LEPR) at MRNA and surface protein levels, which was regulated by IL-33. Leptin exerts strong effects on several basophyl functions. This causes a strong migration response to human basophyl, potentially similar to the basophil-active kemokine. Also, Leptin increases the survival of human basophyl, even though its potential is less than IL-3. In addition, CD63, the marker of basophil activation is stated on the cell surface, regulated by leptin, the effect is neutralized by blocking LEPR.
Basophyl degranulation assessment and cytokine synthesis found that leptin shows a strong priming effect on human basophyl degranulation in response to FCεRI aggregation and induces Th2, but not the production of cytokines by cells. In short, these findings indicate that leptin may be the main molecule that mediates the effects of adipocytes in inflammatory cells such as basophiles by binding LEPR and activating cellular functions, which may worsen allergic inflammation.
Learn at the synthesis of complement components by human kidney mesangial cells in culture. Assessment of cytokine effects.
Cultivation of kidney mesangial cells (MC) is easily established and widely used. MCs produce several complementary regulatory proteins (C). We studied whether MC synthesized components C (C3, C5, C8). MC culture was established from the normal portion of nephrectomy for kidney cancer. After growing to a close meeting in RPMI / 17% FBS and resting 24 hours in RPMI / 0.5% FBS, MC stimulated up to 72 hours with IL-1β or IL-6 (10, 100, 1000 U / mL). Both C5 and C8 detection by ELISA.
While C3 is present in supernatant in basal conditions (15.5-107.6 ng / 10 (6) cells / 24 hours) in different MC lines. IL-1β regulates synthesis with 2.4-4.5 folds, while IL-6 does not show any effect. The c3 synthetic rate is cell 1.76 ng / 10 (6) under IL-1 stimulation versus basal level 0.37 ng / hour / 10 (6) cells. MC C3 production, especially induced by IL-1 may have pathogenetic relevance in glomerulonephritis.
Rooperol, cytokine synthesis inhibitors, reduces breathing explosions on leukocytes and human macrophies and mice.
Chemiluminescence enhanced by Luminol is measured in all fresh human blood, or human neutrophils are isolated from the blood of heparation, macrophages of alveolar humans and macrophages of alveolar rats which are stimulated with endotoxin bacteria (LPS). Esther TetraAceTate from Rooperol, a recatechol that shows anticoytokine activity, added to the cell simultaneously with LPS inhibiting the breathing explosion. The effective concentration of Rooperol is in the range of 1-10 mothers depending on the type of cell and in accordance with the inhibition of the production of nitrate oxide by macrophages of mice alveolar. Thus Roerol can reduce some effects of excessive phagocytic activity and inflammatory reactions but by extinguishing free radical production can also reduce resistance to bacterial infections.
Leptin increases survival and induces migration, degranulation, and synthesis of cytokines from human basophyl.
Basophils are the rarest leukocytes in human blood, but are now recognized as one of the most important immunomodulatory cells and the effector in allergic inflammation. Leptin, a member of the Cytokine IL-6 family, has a metabolic effect as an adipocine, and is also known to participate in the pathogenesis of inflammatory reactions. Because there is an epidemiological relationship between obesity and allergies, we examine whether the basophyl function is modified by leptin. We found that the human basophyl expressed Leptin receptor (LEPR) at MRNA and surface protein levels, which was regulated by IL-33.
Leptin exerts strong effects on several basophyl functions. This causes a strong migration response to human basophyl, potentially similar to the basophil-active kemokine. Also, Leptin increases the survival of human basophyl, even though its potential is less than IL-3. In addition, CD63, the marker of basophil activation is stated on the cell surface, regulated by leptin, the effect is neutralized by blocking LEPR. Basophyl degranulation assessment and cytokine synthesis found that leptin shows a strong priming effect on human basophyl degranulation in response to FCεRI aggregation and induces Th2, but not the production of cytokines by cells.
 Recombinant Human Endoglin |
|
CC026-010 |
GenDepot |
10ug |
EUR 1347.6 |
 Recombinant Human Visfatin |
|
CC143-005 |
GenDepot |
5ug |
EUR 351.6 |
Recombinant Human Visfatin |
|
CC143-025 |
GenDepot |
25ug |
EUR 489.6 |
Recombinant Human Visfatin |
|
CC143-101 |
GenDepot |
1mg |
EUR 10752 |
 Recombinant Human Vaspin |
|
CC152-005 |
GenDepot |
5ug |
EUR 351.6 |
Recombinant Human Vaspin |
|
CC152-025 |
GenDepot |
25ug |
EUR 489.6 |
Recombinant Human Vaspin |
|
CC152-101 |
GenDepot |
1mg |
EUR 10752 |
 Recombinant Human S100A7 |
|
CR28-10ug |
Novoprotein |
10ug |
EUR 187.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Human S100A7 |
|
CR28-1mg |
Novoprotein |
1mg |
EUR 2252.4 |
|
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Human S100A7 |
|
CR28-500ug |
Novoprotein |
500ug |
EUR 1593.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Human S100A7 |
|
CR28-50ug |
Novoprotein |
50ug |
EUR 442.8 |
|
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
 Recombinant Human S100A11 |
|
CR32-10ug |
Novoprotein |
10ug |
EUR 187.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Human S100A11 |
|
CR32-1mg |
Novoprotein |
1mg |
EUR 2252.4 |
|
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Human S100A11 |
|
CR32-500ug |
Novoprotein |
500ug |
EUR 1593.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Recombinant Human S100A11 |
|
CR32-50ug |
Novoprotein |
50ug |
EUR 442.8 |
|
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
 Recombinant Human S100A16 |
|
CR33-10ug |
Novoprotein |
10ug |
EUR 145.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0. |
Recombinant Human S100A16 |
|
CR33-1mg |
Novoprotein |
1mg |
EUR 1521.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0. |
Recombinant Human S100A16 |
|
CR33-500ug |
Novoprotein |
500ug |
EUR 1082.4 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0. |
Recombinant Human S100A16 |
|
CR33-50ug |
Novoprotein |
50ug |
EUR 315.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0. |
 Recombinant Human Artemin |
|
CR34-10ug |
Novoprotein |
10ug |
EUR 169.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
Recombinant Human Artemin |
|
CR34-1mg |
Novoprotein |
1mg |
EUR 2739.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
Recombinant Human Artemin |
|
CR34-500ug |
Novoprotein |
500ug |
EUR 1935.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
Recombinant Human Artemin |
|
CR34-50ug |
Novoprotein |
50ug |
EUR 363.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
 Recombinant Human Neurturin |
|
CE27-10ug |
Novoprotein |
10ug |
EUR 187.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 10mM sodium citrate, pH4.0. |
Recombinant Human Neurturin |
|
CE27-1mg |
Novoprotein |
1mg |
EUR 2739.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 10mM sodium citrate, pH4.0. |
Recombinant Human Neurturin |
|
CE27-500ug |
Novoprotein |
500ug |
EUR 1935.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 10mM sodium citrate, pH4.0. |
Recombinant Human Neurturin |
|
CE27-50ug |
Novoprotein |
50ug |
EUR 442.8 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 10mM sodium citrate, pH4.0. |
 Recombinant Human Leptin |
|
CG069-101 |
GenDepot |
1mg |
EUR 489.6 |
Recombinant Human Leptin |
|
CG069-105 |
GenDepot |
5mg |
EUR 1680 |
Recombinant Human Leptin |
|
CG069-200 |
GenDepot |
200ug |
EUR 351.6 |
 Recombinant Human Myostatin |
|
CG090-002 |
GenDepot |
2ug |
EUR 351.6 |
Recombinant Human Myostatin |
|
CG090-010 |
GenDepot |
10ug |
EUR 489.6 |
Recombinant Human Myostatin |
|
CG090-101 |
GenDepot |
1mg |
EUR 12372 |
 Recombinant Human Noggin |
|
CG137-005 |
GenDepot |
5ug |
EUR 351.6 |
Recombinant Human Noggin |
|
CG137-020 |
GenDepot |
20ug |
EUR 489.6 |
Recombinant Human Noggin |
|
CG137-101 |
GenDepot |
1mg |
EUR 8970 |
 Recombinant Human Persephin |
|
CH91-10ug |
Novoprotein |
10ug |
EUR 169.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
Recombinant Human Persephin |
|
CH91-1mg |
Novoprotein |
1mg |
EUR 2252.4 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
Recombinant Human Persephin |
|
CH91-500ug |
Novoprotein |
500ug |
EUR 1581.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
Recombinant Human Persephin |
|
CH91-50ug |
Novoprotein |
50ug |
EUR 363.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 4mM HCl. |
 Recombinant Human PRL |
|
CM10-10ug |
Novoprotein |
10ug |
EUR 169.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Human PRL |
|
CM10-1mg |
Novoprotein |
1mg |
EUR 2008.8 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Human PRL |
|
CM10-500ug |
Novoprotein |
500ug |
EUR 1423.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Human PRL |
|
CM10-50ug |
Novoprotein |
50ug |
EUR 363.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Recombinant Human Leptin |
|
C067-10ug |
Novoprotein |
10ug |
EUR 90 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Leptin |
|
C067-1mg |
Novoprotein |
1mg |
EUR 303.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Leptin |
|
C067-500ug |
Novoprotein |
500ug |
EUR 230.4 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Recombinant Human Leptin |
|
C067-50ug |
Novoprotein |
50ug |
EUR 120 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
 Lactoferrin, Human Recombinant |
|
CA079-010 |
GenDepot |
10g |
Ask for price |
 Recombinant Human Vinculin |
|
C264-10ug |
Novoprotein |
10ug |
EUR 242.4 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Recombinant Human Vinculin |
|
C264-1mg |
Novoprotein |
1mg |
EUR 2739.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Recombinant Human Vinculin |
|
C264-500ug |
Novoprotein |
500ug |
EUR 1935.6 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Recombinant Human Vinculin |
|
C264-50ug |
Novoprotein |
50ug |
EUR 595.2 |
|
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
 Recombinant Human ACE2 |
|
ACE2-68H-20ug |
Creative BioMart |
20ug |
EUR 684 |
|
Description: Signal peptide sequence and the extracellular domain of human ACE2 (aa 1-740) are fused at the C-terminus to the Fc portion of human IgG1 |
Recombinant Human ACE2 |
|
ACE2-68H-50ug |
Creative BioMart |
50ug |
EUR 996 |
|
Description: Signal peptide sequence and the extracellular domain of human ACE2 (aa 1-740) are fused at the C-terminus to the Fc portion of human IgG2 |
Recombinant Human ACE2 |
|
ACE2-736H-20ug |
Creative BioMart |
20ug |
EUR 684 |
|
Description: Recombinant Human Angiotensin-Converting Enzyme 2/ACE-2 produced by transfected human cells is a secreted protein with sequence (Gln18-Ser740) of human ACE-2 fused with a polyhistidine tag at the C-terminus.The encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. |
 Recombinant Human ACE3 |
|
ACE2-736H-50ug |
Creative BioMart |
50ug |
EUR 996 |
|
Description: Recombinant Human Angiotensin-Converting Enzyme 2/ACE-2 produced by transfected human cells is a secreted protein with sequence (Gln18-Ser740) of human ACE-2 fused with a polyhistidine tag at the C-terminus.The encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. |
 Recombinant Human Adiponectin |
|
7-00019 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Human Adiponectin |
|
7-00020 |
CHI Scientific |
25µg |
Ask for price |
Recombinant Human Adiponectin |
|
7-00021 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Amphiregulin |
|
7-00082 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Amphiregulin |
|
7-00083 |
CHI Scientific |
50µg |
Ask for price |
Recombinant Human Amphiregulin |
|
7-00084 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Betacellulin |
|
7-00223 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Human Betacellulin |
|
7-00224 |
CHI Scientific |
20µg |
Ask for price |
Recombinant Human Betacellulin |
|
7-00225 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Epiregulin |
|
7-00308 |
CHI Scientific |
25µg |
Ask for price |
 Recombinant Human Follistatin |
|
7-00421 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Human Follistatin |
|
7-00422 |
CHI Scientific |
20µg |
Ask for price |
Recombinant Human Follistatin |
|
7-00423 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Myotrophin |
|
7-01274 |
CHI Scientific |
20µg |
Ask for price |
 Recombinant Human Neuroglobin |
|
7-01288 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Neuroglobin |
|
7-01289 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Neuroglobin |
|
7-01290 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Osteoprotegerin |
|
7-01321 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Osteoprotegerin |
|
7-01322 |
CHI Scientific |
50µg |
Ask for price |
Recombinant Human Osteoprotegerin |
|
7-01323 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Progranulin |
|
7-01387 |
CHI Scientific |
2µg |
Ask for price |
Recombinant Human Progranulin |
|
7-01388 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Progranulin |
|
7-01389 |
CHI Scientific |
100µg |
Ask for price |
 Recombinant Human Pleiotrophin |
|
7-01390 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Human Pleiotrophin |
|
7-01391 |
CHI Scientific |
20µg |
Ask for price |
Recombinant Human Pleiotrophin |
|
7-01392 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Calmodulin |
|
7-04657 |
CHI Scientific |
10µg |
Ask for price |
Recombinant Human Calmodulin |
|
7-04658 |
CHI Scientific |
50µg |
Ask for price |
 Recombinant Human Calreticulin |
|
7-04666 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Human Calreticulin |
|
7-04667 |
CHI Scientific |
25µg |
Ask for price |
Recombinant Human Calreticulin |
|
7-04668 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Calcyphosine |
|
7-04693 |
CHI Scientific |
5µg |
Ask for price |
Recombinant Human Calcyphosine |
|
7-04694 |
CHI Scientific |
20µg |
Ask for price |
Recombinant Human Calcyphosine |
|
7-04695 |
CHI Scientific |
1mg |
Ask for price |
 Recombinant Human Cytoglobin |
|
7-04955 |
CHI Scientific |
10µg |
Ask for price |
In short, these findings indicate that leptin may be the main molecule that mediates the effects of adipocytes in inflammatory cells such as basophiles by binding LEPR and activating cellular functions, which may worsen allergic inflammation.
