Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen.

Description: The Mouse PDE2A Assay Kit is designed for identification of inhibitors of Mouse PDE2A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE2A to the binding agent._x000D_The key to the Mouse PDE2A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE2A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE2A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: The Mouse PDE7B Assay Kit is designed for identification of inhibitors of Mouse PDE7B using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE7B to the binding agent. The key to the Mouse PDE7B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE7B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE7B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Mouse PDE1B, also known as cAMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility._x000D_The Mouse PDE1B Assay Kit is designed for identification of inhibitors of Mouse PDE1B using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE1B to the binding agent._x000D_Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearlypolarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light._x000D_The key to the Mouse PDE1B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE1B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE1B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: Phosphodiesterases (PDEs) play an important role in dynamic_x000D_regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is_x000D_expressed principally in human myocardium. The key to the PDE1C (Mouse) Assay Kit is the_x000D_specific binding agent. Using this kit, only two simple steps on a microtiter plate are required_x000D_for PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a sample_x000D_containing PDE1C for 1 hour. Second, a binding agent is added to the reaction mix to_x000D_produce a change in fluorescent polarization that can then be measured using a_x000D_fluorescence reader equipped for the measurement of fluorescence polarization._x000D_

Description: Minimum order quantity: 1 unit of 1KIT

Description: The IDO1 Inhibitor Mechanism of Action (MoA) Assay Kit is designed to_x000D_determine the mechanism of IDO1 enzyme inhibition (e.g., reversible or irreversible inhibition)._x000D_The IDO1 Mechanism of Action Assay Kit is simple to use. Inhibitor and_x000D_enzyme are preincubated allowing the inhibitor to bind. After preincubation, the mixture is_x000D_diluted into reaction buffer containing the L-tryptophan (L-Trp) substrate and all necessary_x000D_coupled reaction components. This allows reversible inhibitors to dissociate, while irreversible_x000D_inhibitors remain associated with the IDO1 enzyme. IDO1 activity is determined by measuring_x000D_the absorption of the reaction product at
=320 - 325 nm.

Description: A sandwich ELISA for quantitative measurement of Mouse Fibrinogen β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A sandwich ELISA for quantitative measurement of Mouse Fibrinogen β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A sandwich ELISA for quantitative measurement of Mouse Fibrinogen β in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fibrinogen (FG) in plasma.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fibrinogen (FG) in plasma.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fibrinogen (FG) in plasma.

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fibrinogen (FG) in plasma.

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Fibrinogen (FG) in samples from plasma with no significant corss-reactivity with analogues from other species.