AIMS / HYPOTHESIS
inflammatory signals and increase the synthesis of prostaglandins play a role during the development of diabetes. The prostaglandin D2 (PGD2) receptor, GPR44 / DP2, is highly expressed in human islets and insulin secretion pathway activation results impaired. GPR44 activation role in the function of small islands and the survival rate for chronic hyperglycaemic conditions are unknown. In this study, we investigated GPR44 GPR44 inhibition by using a selective antagonist (AZ8154) in humans the island both in vitro and in vivo in diabetic mice transplanted with human islets.

METHOD
human islets exposed to PGD2 or proinflammatory cytokines in vitro to determine the effect of GPR44 inhibition on the survival rate of the island. In addition, the molecular mechanism of inhibition of GPR44 investigated in human islets exposed to high concentrations of glucose (HG) and IL-1β.

To the in vivo from this study, human islets are transplanted under the kidney capsule of diabetic rats immunodeficiencies and treated with 6, 60 or 100 mg / kg per day antagonist of GPR44 from the day of transplantation until day 4 short-term (studies) or study days (term long-17) post transplant. IVGTT conducted in rats at 10 days 15 days post transplant. After the termination of the study, metabolic variables, human circulating proinflammatory cytokines, and hepatocyte growth factor (HGF) were analyzed in human islets transplanted.

RESULTS
PGD2 or proinflammatory cytokine-induced apoptosis in human islets, while GPR44 inhibition of reverse these effects. GPR44 inhibition of hate decreased insulin secretion induced by glucose-stimulated HG and IL-1β in human islets. It is accompanied by activation of glycogen synthase kinase-Akt signaling 3β together with the phosphorylation and inactivation of the forkhead box O-1 and upregulation of pancreatic and duodenal homeobox-1 and HGF.

GPR44 antagonist administration up to 17 days for the diabetic mice transplanted with human islets resulted in a marginal reduction in fasting blood glucose and lower glucose fluctuations during IVGTT. improved glucose regulation is supported by an increased level of human C-peptide compared to the vehicle group on day 4 and throughout the treatment period. GPR44 inhibition reduces plasma levels of TNF-α and growth-regulated oncogene-α / chemokine (C-X-C motif) ligand 1 and raise the level of HGF in human islets.

Inhibition of GPR44 in human islets have the potential to improve the functioning of small islands and the survival rate under pressure inflammation and hyperglycaemic. This may have implications for the survival rates were better than the island after transplantation.

1 Channel Control Pannexin endothelial inflammation by regulating intracellular calcium.

The proinflammatory cytokine IL-1β is a significant risk factor in cardiovascular disease that can be targeted to reduce major cardiovascular events. expression and release of IL-1β is tightly controlled by changes in intracellular Ca2 + ([Ca2 +] i), which has been associated with the release of ATP and purinergic signaling. However, the mechanisms that regulate these changes have not been identified.

Interleukin 8 (IL8) Antibody
20-abx100964	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx100965	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx100966	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx100967	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx100969	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx100970	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx100971	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx100972	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131832	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131833	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131834	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131835	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131836	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131837	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131838	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131839	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx131840	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx137023	Abbexa	Ask for price Ask for price	0.5 mg 1 mg

Interleukin 8 (IL8) Antibody
abx036426-100ug	Abbexa	100 ug	EUR 469.2

Interleukin 8 (IL8) Antibody
abx018002-100ug	Abbexa	100 ug	EUR 410.4

Interleukin 8 (IL8) Antibody
abx020956-05mg	Abbexa	0.5 mg	EUR 1296

Interleukin 8 (IL8) Antibody
20-abx009111	Abbexa	Ask for price Ask for price Ask for price	100 ul 200 ul 30 ul

Interleukin 8 (IL8) Antibody
20-abx270103	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 tests 200 tests 25 tests 500 tests 50 tests

Interleukin 8 (IL8) Antibody
20-abx270152	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 tests 200 tests 25 tests 500 tests 50 tests

Interleukin 8 (IL8) Antibody
20-abx270200	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 tests 200 tests 25 tests 500 tests 50 tests

Interleukin 8 (IL8) Antibody
20-abx270201	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 tests 200 tests 25 tests 500 tests 50 tests

Interleukin 8 (IL8) Antibody
20-abx173191	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Interleukin 8 (IL8) Antibody
abx216289-100ug	Abbexa	100 ug	EUR 526.8

Interleukin 8 (IL8) Antibody
20-abx177199	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Interleukin 8 (IL8) Antibody
20-abx177201	Abbexa	Ask for price Ask for price	1 mg 200 ug

Interleukin 8 (IL8) Antibody
abx173191-100l	Abbexa	100 µl	EUR 287.5

Interleukin 8 (IL8) Antibody
abx173191-1ml	Abbexa	1 ml	EUR 850

Interleukin 8 (IL8) Antibody
abx173191-200l	Abbexa	200 µl	EUR 375

Interleukin 8 (IL8) Antibody
abx177199-1096tests	Abbexa	10 × 96 tests	EUR 625

Interleukin 8 (IL8) Antibody
abx177199-596tests	Abbexa	5 × 96 tests	EUR 300

Interleukin 8 (IL8) Antibody
abx036426-100g	Abbexa	100 µg	EUR 337.5

Interleukin 8 (IL8) Antibody
abx018002-1mg	Abbexa	1 mg	EUR 262.5

Interleukin 8 (IL8) Antibody
abx270152-1ml	Abbexa	1 ml	EUR 425

Interleukin 8 (IL8) Antibody
abx270152-200l	Abbexa	200 µl	EUR 300

Interleukin 8 (IL8) Antibody
abx131832-100g	Abbexa	100 µg	EUR 875

Interleukin 8 (IL8) Antibody
abx131832-20g	Abbexa	20 µg	EUR 375

Interleukin 8 (IL8) Antibody
abx131832-5g	Abbexa	5 µg	EUR 300

Interleukin 8 (IL8) Antibody
abx131833-100g	Abbexa	100 µg	EUR 812.5

Interleukin 8 (IL8) Antibody
abx131833-20g	Abbexa	20 µg	EUR 362.5

Interleukin 8 (IL8) Antibody
abx131833-5g	Abbexa	5 µg	EUR 287.5

Interleukin 8 (IL8) Antibody
abx131834-100g	Abbexa	100 µg	EUR 762.5

Interleukin 8 (IL8) Antibody
abx131834-20g	Abbexa	20 µg	EUR 337.5

Interleukin 8 (IL8) Antibody
abx131834-5g	Abbexa	5 µg	EUR 275

Interleukin 8 (IL8) Antibody
abx131835-100g	Abbexa	100 µg	EUR 775

Interleukin 8 (IL8) Antibody
abx131835-20g	Abbexa	20 µg	EUR 350

Interleukin 8 (IL8) Antibody
abx131835-5g	Abbexa	5 µg	EUR 275

Interleukin 8 (IL8) Antibody
abx131836-100g	Abbexa	100 µg	EUR 812.5

Interleukin 8 (IL8) Antibody
abx131836-20g	Abbexa	20 µg	EUR 362.5

Interleukin 8 (IL8) Antibody
abx131836-5g	Abbexa	5 µg	EUR 287.5

Interleukin 8 (IL8) Antibody
abx131837-100g	Abbexa	100 µg	EUR 812.5

Interleukin 8 (IL8) Antibody
abx131837-20g	Abbexa	20 µg	EUR 362.5

Interleukin 8 (IL8) Antibody
abx131837-5g	Abbexa	5 µg	EUR 287.5

Interleukin 8 (IL8) Antibody
abx131838-100g	Abbexa	100 µg	EUR 625

Interleukin 8 (IL8) Antibody
abx131838-20g	Abbexa	20 µg	EUR 300

Interleukin 8 (IL8) Antibody
abx131838-5g	Abbexa	5 µg	EUR 237.5

Interleukin 8 (IL8) Antibody
abx131839-100g	Abbexa	100 µg	EUR 762.5

Interleukin 8 (IL8) Antibody
abx131839-20g	Abbexa	20 µg	EUR 337.5

Interleukin 8 (IL8) Antibody
abx131839-5g	Abbexa	5 µg	EUR 275

Interleukin 8 (IL8) Antibody
abx131840-100g	Abbexa	100 µg	EUR 762.5

Interleukin 8 (IL8) Antibody
abx131840-20g	Abbexa	20 µg	EUR 337.5

Interleukin 8 (IL8) Antibody
abx131840-5g	Abbexa	5 µg	EUR 275

Interleukin 8 (IL8) Antibody
abx137023-01mg	Abbexa	0.1 mg	EUR 487.5

Interleukin 8 (IL8) Antibody
abx177199-96tests	Abbexa	96 tests	EUR 237.5

Interleukin 8 (IL8) Antibody
abx100960-100l	Abbexa	100 µl	EUR 212.5

Interleukin 8 (IL8) Antibody
abx100960-1ml	Abbexa	1 ml	EUR 475

Interleukin 8 (IL8) Antibody
abx100960-200l	Abbexa	200 µl	EUR 250

Interleukin 8 (IL8) Antibody
abx100961-100l	Abbexa	100 µl	EUR 250

Interleukin 8 (IL8) Antibody
abx100961-1ml	Abbexa	1 ml	EUR 687.5

Interleukin 8 (IL8) Antibody
abx100961-200l	Abbexa	200 µl	EUR 325

Interleukin 8 (IL8) Antibody
abx100962-100l	Abbexa	100 µl	EUR 250

Interleukin 8 (IL8) Antibody
abx100962-1ml	Abbexa	1 ml	EUR 687.5

Interleukin 8 (IL8) Antibody
abx100962-200l	Abbexa	200 µl	EUR 325

Interleukin 8 (IL8) Antibody
abx100963-100l	Abbexa	100 µl	EUR 237.5

Interleukin 8 (IL8) Antibody
abx100963-1ml	Abbexa	1 ml	EUR 625

Interleukin 8 (IL8) Antibody
abx100963-200l	Abbexa	200 µl	EUR 300

Interleukin 8 (IL8) Antibody
abx100964-100l	Abbexa	100 µl	EUR 237.5

Interleukin 8 (IL8) Antibody
abx100964-1ml	Abbexa	1 ml	EUR 625

Interleukin 8 (IL8) Antibody
abx100964-200l	Abbexa	200 µl	EUR 300

Interleukin 8 (IL8) Antibody
abx100965-100l	Abbexa	100 µl	EUR 250

Interleukin 8 (IL8) Antibody
abx100965-1ml	Abbexa	1 ml	EUR 650

Interleukin 8 (IL8) Antibody
abx100965-200l	Abbexa	200 µl	EUR 312.5

Interleukin 8 (IL8) Antibody
abx100966-100l	Abbexa	100 µl	EUR 262.5

Interleukin 8 (IL8) Antibody
abx100966-1ml	Abbexa	1 ml	EUR 687.5

Interleukin 8 (IL8) Antibody
abx100966-200l	Abbexa	200 µl	EUR 325

Interleukin 8 (IL8) Antibody
abx100967-100l	Abbexa	100 µl	EUR 262.5

Interleukin 8 (IL8) Antibody
abx100967-1ml	Abbexa	1 ml	EUR 712.5

Interleukin 8 (IL8) Antibody
abx100967-200l	Abbexa	200 µl	EUR 325

Interleukin 8 (IL8) Antibody
abx100969-100l	Abbexa	100 µl	EUR 275

Interleukin 8 (IL8) Antibody
abx100969-1ml	Abbexa	1 ml	EUR 775

Interleukin 8 (IL8) Antibody
abx100969-200l	Abbexa	200 µl	EUR 350

Interleukin 8 (IL8) Antibody
abx100971-100l	Abbexa	100 µl	EUR 287.5

Interleukin 8 (IL8) Antibody
abx100971-1ml	Abbexa	1 ml	EUR 825

Interleukin 8 (IL8) Antibody
abx100971-200l	Abbexa	200 µl	EUR 362.5

Interleukin 8 (IL8) Antibody
abx100972-100l	Abbexa	100 µl	EUR 300

Interleukin 8 (IL8) Antibody
abx100972-1ml	Abbexa	1 ml	EUR 875

Interleukin 8 (IL8) Antibody
abx100972-200l	Abbexa	200 µl	EUR 375

IL8 (untagged)-Human interleukin 8 (IL8)
SC119807	Origene Technologies GmbH	10 µg	Ask for price

Dog Interleukin-8 (IL8)
1-CSB-YP011671DO	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Dog Interleukin-8(IL8) expressed in Yeast

Dog Interleukin-8 (IL8)
1-CSB-EP011671DO	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Dog Interleukin-8(IL8) expressed in E.coli

Dog Interleukin-8 (IL8)
1-CSB-MP011671DO	Cusabio	Ask for price Ask for price Ask for price Ask for price	100ug 1MG 200ug 500ug
Description: Recombinant Dog Interleukin-8(IL8) expressed in Mammalian cell

Active Interleukin 8 (IL8)
APA080Bo61	Cloud-Clone	10ug	EUR 302

Active Interleukin 8 (IL8)
APA080Ga01	Cloud-Clone	10ug	EUR 346

Active Interleukin 8 (IL8)
APA080Hu01	Cloud-Clone	10ug	EUR 147

Active Interleukin 8 (IL8)
4-APA080Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Interleukin 8 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 8 (IL8)
APA080Po01	Cloud-Clone	10ug	EUR 346

Active Interleukin 8 (IL8)
APA080Rb01	Cloud-Clone	10ug	EUR 346

Active Interleukin 8 (IL8)
APA080Si01	Cloud-Clone	10ug	EUR 346

Sheep Interleukin-8 (IL8)
1-CSB-EP011671SH	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Sheep Interleukin-8(IL8) expressed in E.coli

Active Interleukin 8 (IL8)
RPU55475-100ug	Biomatik Corporation	100ug	EUR 453.2

Active Interleukin 8 (IL8)
RPU55475-1mg	Biomatik Corporation	1mg	EUR 2009.8

Active Interleukin 8 (IL8)
RPU55475-50ug	Biomatik Corporation	50ug	EUR 364.1

Active Interleukin 8 (IL8)
RPU59242-100ug	Biomatik Corporation	100ug	EUR 1140.2

Active Interleukin 8 (IL8)
RPU59242-1mg	Biomatik Corporation	1mg	EUR 5054.4

Active Interleukin 8 (IL8)
RPU59242-50ug	Biomatik Corporation	50ug	EUR 760.3

Active Interleukin 8 (IL8)
MBS2122275-001mg	MyBiosource	0.01mg	EUR 260

Active Interleukin 8 (IL8)
MBS2122275-005mg	MyBiosource	0.05mg	EUR 555

Active Interleukin 8 (IL8)
MBS2122275-01mg	MyBiosource	0.1mg	EUR 850

Active Interleukin 8 (IL8)
MBS2122275-02mg	MyBiosource	0.2mg	EUR 1055

Active Interleukin 8 (IL8)
MBS2122275-05mg	MyBiosource	0.5mg	EUR 2050

Active Interleukin 8 (IL8)
MBS2105072-001mg	MyBiosource	0.01mg	EUR 160

Active Interleukin 8 (IL8)
MBS2105072-005mg	MyBiosource	0.05mg	EUR 265

Active Interleukin 8 (IL8)
MBS2105072-01mg	MyBiosource	0.1mg	EUR 395

Active Interleukin 8 (IL8)
MBS2105072-02mg	MyBiosource	0.2mg	EUR 480

The pannexin 1 (Panx1) channel has been canonically been involved in the ATP release, particularly during inflammation. We checked Panx1 in human umbilical vein endothelial cells after treatment with proinflammatory cytokines TNF-α. Analysis by the whole transcriptome sequencing and immunoblot Panx1 identified a dramatic increase in mRNA and protein expression were regulated by NF-kB-dependent.