Antibodies, Assay Kits, Biology Cells, cDNA, Clia Kits, Culture Cells, Devices, DNA, DNA Templates, Elisa Kits, Enzymes, Equipments, Exosomes, Gels, Isotypes, Medium & Serums, NATtrol, Panel, Particles, PCR, Pcr Kits, Peptides, Reagents, Recombinant Proteins, Ria Kits, RNA, Test Kits, Vector & Virus, Western Blot

Autophagy modulation altered differentiation capacity of CD146+ cells toward endothelial cells, pericytes, and cardiomyocytes.

Autophagy modulation altered differentiation capacity of CD146+ cells toward endothelial cells, pericytes, and cardiomyocytes.
Posted by Frank

BACKGROUND
Until now, a lot of effort used to enhance the regenerative potential of stem cells. In this study, we evaluated the hypothesis whether the modulation of autophagy could change differentiation potential of CD146 + cells into mature pericytes, endothelium, and cardiomyocytes descent.


METHOD
In this study, CD146 + cells enriched from human bone marrow aspiration and trans-differentiated into mature endothelial cells, pericytes, and cardiomyocytes after exposure autophagy stimulator (50 pM Met) / inhibitor (15-pM HCQ). Protein autophagy protein levels are monitored by western blotting.

NO content measured using the Griess test. Using real-time PCR assay and western blotting, we monitor the rate of descent of proteins and genes. Pro-inflammatory cytokines and angiocrine factors were measured by ELISA. Changes in fatty acids determined by gas chromatography. We also measure exosome secretion capacity by measuring the activity of AChE and real-time PCR.


RESULTS
Data revealed the factors modulating autophagy, Beclin-1, P62, and the ratio of LC3-II / I in CD146 + cells differentiate after exposure to the Met and HCQ (p <0.05). Inhibition of autophagy increased NO content compared with Met-treated cells (p <0.05). Real-time PCR analysis showed that treatment with CD146 + cells autophagy modulators alter expression of VE-cadherin, cTnI, and α-SMA (p <0.05). Met increasing the expression of VE-cadherin, α-SMA, and cTnI compared to HCQ-treated cells (p <0.05), while western blotting revealed the protein synthesis of all proteins specific line under the stimulation and inhibition of autophagy.

No statistically significant difference was found in levels of Tie-1, Tie-2, VEGFR-1 and VEGFR-2 after autophagy modulation. Fatty acid profile analysis revealed an increase in polyunsaturated fatty acids after exposure HCQ (p <0.05). Treatment of cells with HCQ increase levels of TNF-α and IL-6 compared with Met-treated cells. The data revealed an increase exosome biogenesis and secretion to the supernatant in cells treated with HCQ compared with Met group (p <0.05).


CONCLUSION
In short, the modulation of autophagy could change differentiation potential of CD146 + cells that are important in cardiac regeneration.

Autophagy modulation altered differentiation capacity of CD146+ cells toward endothelial cells, pericytes, and cardiomyocytes.
Autophagy modulation altered differentiation capacity of CD146+ cells toward endothelial cells, pericytes, and cardiomyocytes.

Iron Deprivation in Human T Cells Induces Nonproliferating Accessories Helper Sel.

Iron uptake via the transferrin receptor (CD71) is an important mechanism for the proliferation of T cells, however, it is not fully understood if targeting CD71 also affect the differentiation and functional polarization of primary human T cells.

In this study, we demonstrated that inhibition of iron consumption by blocking nonproliferating CD71 mAbs to induce T cell, which releases high amounts of IL-2. The target of CD71 by blocking or nonblocking mAbs did not alter the main signal path and the activation of the transcription factor NF-kB, NFAT, or AP-1 as analyzed in Jurkat T cell growth arrest in T cells were deficient in iron (Fe-def) prevented after the addition of exogenous iron in the form of ferric ammonium citrate but not reversible by exogenous IL-2.

Interleukin 6 (IL6) Antibody

abx019116-100ug 100 ug
EUR 342

Bovine Interleukin 6 (IL6) ELISA Kit

DLR-IL6-b-48T 48T
EUR 493
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Interleukin 6 (IL6) ELISA Kit

DLR-IL6-b-96T 96T
EUR 641
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Interleukin 6 (IL6) ELISA Kit

DLR-IL6-c-48T 48T
EUR 474
Description: A sandwich quantitative ELISA assay kit for detection of Canine Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Interleukin 6 (IL6) ELISA Kit

DLR-IL6-c-96T 96T
EUR 614
Description: A sandwich quantitative ELISA assay kit for detection of Canine Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Chicken Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Ch-48T 48T
EUR 454
Description: A sandwich quantitative ELISA assay kit for detection of Chicken Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Chicken Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Ch-96T 96T
EUR 587
Description: A sandwich quantitative ELISA assay kit for detection of Chicken Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Eq-48T 48T
EUR 503
Description: A sandwich quantitative ELISA assay kit for detection of Equine Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Equine Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Eq-96T 96T
EUR 654
Description: A sandwich quantitative ELISA assay kit for detection of Equine Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Interleukin 6 (IL6) ELISA Kit

DLR-IL6-g-48T 48T
EUR 456
Description: A sandwich quantitative ELISA assay kit for detection of Goat Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Goat Interleukin 6 (IL6) ELISA Kit

DLR-IL6-g-96T 96T
EUR 590
Description: A sandwich quantitative ELISA assay kit for detection of Goat Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Hu-48T 48T
EUR 350
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Hu-96T 96T
EUR 444
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Mu-48T 48T
EUR 357
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Mu-96T 96T
EUR 454
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Interleukin 6 (IL6) ELISA Kit

DLR-IL6-p-48T 48T
EUR 493
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Interleukin 6 (IL6) ELISA Kit

DLR-IL6-p-96T 96T
EUR 641
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Ra-48T 48T
EUR 372
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Ra-96T 96T
EUR 475
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Rb-48T 48T
EUR 454
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rabbit Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Rb-96T 96T
EUR 587
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Sheep Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Sh-48T 48T
EUR 522
Description: A sandwich quantitative ELISA assay kit for detection of Sheep Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Sheep Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Sh-96T 96T
EUR 681
Description: A sandwich quantitative ELISA assay kit for detection of Sheep Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Monkey Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Si-48T 48T
EUR 522
Description: A sandwich quantitative ELISA assay kit for detection of Monkey Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Monkey Interleukin 6 (IL6) ELISA Kit

DLR-IL6-Si-96T 96T
EUR 681
Description: A sandwich quantitative ELISA assay kit for detection of Monkey Interleukin 6 (IL6) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Interleukin 6 (IL6) ELISA Kit

RDR-IL6-b-48Tests 48 Tests
EUR 516

Bovine Interleukin 6 (IL6) ELISA Kit

RDR-IL6-b-96Tests 96 Tests
EUR 716

Canine Interleukin 6 (IL6) ELISA Kit

RDR-IL6-c-48Tests 48 Tests
EUR 493

Canine Interleukin 6 (IL6) ELISA Kit

RDR-IL6-c-96Tests 96 Tests
EUR 683

Chicken Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Ch-48Tests 48 Tests
EUR 470

Chicken Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Ch-96Tests 96 Tests
EUR 651

Equine Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Eq-48Tests 48 Tests
EUR 528

Equine Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Eq-96Tests 96 Tests
EUR 733

Goat Interleukin 6 (IL6) ELISA Kit

RDR-IL6-g-48Tests 48 Tests
EUR 473

Goat Interleukin 6 (IL6) ELISA Kit

RDR-IL6-g-96Tests 96 Tests
EUR 654

Human Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Hu-48Tests 48 Tests
EUR 347

Human Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Hu-96Tests 96 Tests
EUR 474

Mouse Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Mu-48Tests 48 Tests
EUR 356

Mouse Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Mu-96Tests 96 Tests
EUR 487

Porcine Interleukin 6 (IL6) ELISA Kit

RDR-IL6-p-48Tests 48 Tests
EUR 516

Porcine Interleukin 6 (IL6) ELISA Kit

RDR-IL6-p-96Tests 96 Tests
EUR 716

Rat Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Ra-48Tests 48 Tests
EUR 374

Rat Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Ra-96Tests 96 Tests
EUR 512

Rabbit Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Rb-48Tests 48 Tests
EUR 470

Rabbit Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Rb-96Tests 96 Tests
EUR 651

Sheep Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Sh-48Tests 48 Tests
EUR 551

Sheep Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Sh-96Tests 96 Tests
EUR 765

Monkey Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Si-48Tests 48 Tests
EUR 551

Monkey Interleukin 6 (IL6) ELISA Kit

RDR-IL6-Si-96Tests 96 Tests
EUR 765

Bovine Interleukin 6 (IL6) ELISA Kit

RD-IL6-b-48Tests 48 Tests
EUR 494

Bovine Interleukin 6 (IL6) ELISA Kit

RD-IL6-b-96Tests 96 Tests
EUR 684

Canine Interleukin 6 (IL6) ELISA Kit

RD-IL6-c-48Tests 48 Tests
EUR 472

Canine Interleukin 6 (IL6) ELISA Kit

RD-IL6-c-96Tests 96 Tests
EUR 653

Chicken Interleukin 6 (IL6) ELISA Kit

RD-IL6-Ch-48Tests 48 Tests
EUR 450

Chicken Interleukin 6 (IL6) ELISA Kit

RD-IL6-Ch-96Tests 96 Tests
EUR 622

Equine Interleukin 6 (IL6) ELISA Kit

RD-IL6-Eq-48Tests 48 Tests
EUR 505

Equine Interleukin 6 (IL6) ELISA Kit

RD-IL6-Eq-96Tests 96 Tests
EUR 700

Goat Interleukin 6 (IL6) ELISA Kit

RD-IL6-g-48Tests 48 Tests
EUR 453

Goat Interleukin 6 (IL6) ELISA Kit

RD-IL6-g-96Tests 96 Tests
EUR 625

Human Interleukin 6 (IL6) ELISA Kit

RD-IL6-Hu-48Tests 48 Tests
EUR 333

Human Interleukin 6 (IL6) ELISA Kit

RD-IL6-Hu-96Tests 96 Tests
EUR 454

Mouse Interleukin 6 (IL6) ELISA Kit

RD-IL6-Mu-48Tests 48 Tests
EUR 341

Mouse Interleukin 6 (IL6) ELISA Kit

RD-IL6-Mu-96Tests 96 Tests
EUR 466

Porcine Interleukin 6 (IL6) ELISA Kit

RD-IL6-p-48Tests 48 Tests
EUR 494

Porcine Interleukin 6 (IL6) ELISA Kit

RD-IL6-p-96Tests 96 Tests
EUR 684

Rat Interleukin 6 (IL6) ELISA Kit

RD-IL6-Ra-48Tests 48 Tests
EUR 358

Rat Interleukin 6 (IL6) ELISA Kit

RD-IL6-Ra-96Tests 96 Tests
EUR 490

Rabbit Interleukin 6 (IL6) ELISA Kit

RD-IL6-Rb-48Tests 48 Tests
EUR 450

Rabbit Interleukin 6 (IL6) ELISA Kit

RD-IL6-Rb-96Tests 96 Tests
EUR 622

Sheep Interleukin 6 (IL6) ELISA Kit

RD-IL6-Sh-48Tests 48 Tests
EUR 527

Sheep Interleukin 6 (IL6) ELISA Kit

RD-IL6-Sh-96Tests 96 Tests
EUR 731

Monkey Interleukin 6 (IL6) ELISA Kit

RD-IL6-Si-48Tests 48 Tests
EUR 527

Monkey Interleukin 6 (IL6) ELISA Kit

RD-IL6-Si-96Tests 96 Tests
EUR 731

Recombinant Mouse IL6/ Interleukin-6 Protein, Untagged, E.coli-100ug

QP1487-100ug 100ug
EUR 608

Recombinant Human IL6/ Interleukin-6 Protein, Untagged, E.coli-100ug

QP5235-100ug 100ug
EUR 490

Recombinant Rat IL6/ Interleukin-6 Protein, Untagged, E.coli-100ug

QP5501-100ug 100ug
EUR 708

Recombinant Human IL6/ Interleukin 6 Protein, Native, Human-100ug

QDX53-100ug 100ug
EUR 615

Human Interleukin 6 (IL6) Protein

abx060189-100ug 100 ug
EUR 523

Human Interleukin 6 (IL6) Protein

abx060253-100ug 100 ug
EUR 523

Recombinant Rabbit IL6/ Interleukin-6 Protein Protein, His, Yeast-100ug

QP9889-ye-100ug 100ug
EUR 788

Recombinant Human IL6/ Interleukin-6 Protein Protein, Untagged, E.coli-100ug

QP10313-ec-100ug 100ug
EUR 988

Recombinant Mouse IL6/ Interleukin-6 Protein Protein, Untagged, E.coli-100ug

QP10455-ec-100ug 100ug
EUR 988

Recombinant Macaque IL6/ Interleukin-6 Protein Protein, Untagged, E.coli-100ug

QP1556-ec-100ug 100ug
EUR 1678

Recombinant Sheep IL6/ Interleukin-6 Protein Protein, GST, E.coli-100ug

QP6225-ec-100ug 100ug
EUR 707

Recombinant Sheep IL6/ Interleukin-6 Protein Protein, His, Yeast-100ug

QP6225-ye-100ug 100ug
EUR 788

Interleukin 6 (IL6) Antibody

20-abx131321
  • EUR 328.00
  • EUR 133.00
  • EUR 899.00
  • EUR 453.00
  • EUR 286.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 6 (IL6) Antibody

20-abx131322
  • EUR 453.00
  • EUR 133.00
  • EUR 1288.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 6 (IL6) Antibody

20-abx131323
  • EUR 342.00
  • EUR 133.00
  • EUR 996.00
  • EUR 495.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 6 (IL6) Antibody

20-abx131324
  • EUR 328.00
  • EUR 133.00
  • EUR 899.00
  • EUR 453.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 6 (IL6) Antibody

20-abx131986
  • EUR 300.00
  • EUR 704.00
  • EUR 356.00
  • EUR 154.00
  • EUR 244.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Interleukin 6 (IL6) Antibody

20-abx131987
  • EUR 328.00
  • EUR 843.00
  • EUR 439.00
  • EUR 154.00
  • EUR 258.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Interleukin 6 (IL6) Antibody

20-abx131988
  • EUR 328.00
  • EUR 843.00
  • EUR 439.00
  • EUR 154.00
  • EUR 258.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Interleukin 6 (IL6) Antibody

20-abx131989
  • EUR 356.00
  • EUR 954.00
  • EUR 481.00
  • EUR 154.00
  • EUR 272.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Interleukin 6 (IL6) Antibody

20-abx132326
  • EUR 342.00
  • EUR 871.00
  • EUR 453.00
  • EUR 154.00
  • EUR 272.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Surprisingly, the protein synthesis was found intact in the Fe-def T cells as indicated by the level of comparable CD69 upregulation and cytokine production by T cells enough iron to stimulation with CD3 plus CD28 mAbs. Indeed, the high number of IL-2 was detected in the T cell supernatant Fe-def, which is accompanied by reduced cell surface expression of IL-2R. When we use the Fe-def T cells as in MLRS allogeneic, we observed that these cells acquire the accessory cell function and stimulates the proliferation of bystander T cells by providing IL-2

Related Post

Leave A Comment