Bromodomain-containing protein 4 inhibition alleviates matrix degradation by enhancing autophagy and suppressing NLRP3 inflammasome activity in NP cells.

Imbalance between matrix synthesis and degradation is the hallmark of the degeneration of the intervertebral disc while contributing inflammatory cytokine imbalance. Bromodomain and extra-terminal domain (BET) family associated with the pathogenesis of inflammation, and inhibition of BRD4, an important member of the family BET, play an anti-inflammatory role in many diseases.

However, it remains elusive whether BRD4 play the same role in the nucleus pulposus (NP) cells and participates in the pathogenesis of intervertebral disc degeneration. The purpose of this study to examine whether inhibition of BRD4 matrix regulate metabolism by controlling autophagy and NLRP3 inflammasome activity. In addition, it investigated the relationship between nuclear factor kB (NF-kB) signaling, autophagy and NLRP3 inflammasome in NP cells.

Here, real-time polymerase chain reaction, Western blot analysis and GFP-LC3 adenoviral vector transduction in vitro were used, and it was revealed that BRD4 inhibition of matrix degradation and enhance ease of autophagy in the presence or absence of tumor necrosis factor α. In addition, p65 knockdown or treatment with JQ1 and Bay11-7082 indicate that inhibition of BRD4 NLRP3 inflammasome activity weakened through NF-kB signaling, while autophagy inhibition by bafilomycin A1 promoted matrix degradation and cell activity in the NP NLRP3 inflammasome.

In addition, the analysis of RNA expression in tissues BRD4 human NP further verified BRD4 destructive functions. Simply, BRD4 inhibition of matrix degradation Easing by increasing autophagy and suppress the activity of NLRP3 inflammasome through the NF-kB signaling in cells NP.

Bromodomain-containing protein 4 inhibition alleviates matrix degradation by enhancing autophagy and suppressing NLRP3 inflammasome activity in NP cells.
Bromodomain-containing protein 4 inhibition alleviates matrix degradation by enhancing autophagy and suppressing NLRP3 inflammasome activity in NP cells.

Links N Pressing interleukin-1β-induced biological effects in human osteoarthritic cartilage.

Osteoarthritis (OA) is a diarthrodial joint disease associated with proteolytic degradation of extracellular matrix under conditions of inflammation, pain and disability. Currently, no therapies to prevent, reverse or modulate the course of disease. This study aimed to evaluate the potential of regenerative Link N (LN) in human OA cartilage in inflammatory environment and determine whether the LN can influence the behavior associated pain in OA knee injury model rats. Osteo-chondro explants OA and OA chondrocytes treated with LN in the presence of interleukin-1β (IL-1β) to simulate the environment of osteoarthritic.

Von Frey quantitative polymerase chain reaction and Western blotting was performed to determine the effect of LN on matrix protein synthesis, catabolic enzymes, cytokines and nerve growth factor expression. Partial medial meniscectomy (PMM) was performed on the knee C57BL / 6 mice and, 12 weeks post-surgery, the rats were given 5 mg of intra-articular injection of LN or phosphate-buffered saline. A von Frey test carried out for 24 hours to measure the mechanical allodynia in the hind legs.

LN modulated proteoglycans and collagen synthesis in human OA cartilage by inhibiting the biological effects of IL-1β-induced. LN also suppressed the upregulation of IL-1β-induced cartilage-degrading enzymes and inflammatory molecules in OA chondrocytes. After an investigation of the canonical signaling pathway of IL-1β and nuclear factor kappa-light-chain-enhancer activated B cells (NF-kB), LN resulted in significantly inhibit NF-kB activation in a dose-dependent manner.

Interleukin 35 (IL35) Antibody

20-abx129830
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Active Interleukin 35 (IL35)

4-APC008Hu01
  • EUR 657.60
  • EUR 300.00
  • EUR 2136.00
  • EUR 792.00
  • EUR 1464.00
  • EUR 516.00
  • EUR 5160.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 35 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 35 (IL35)

RPU54947-100ug 100ug
EUR 616

Active Interleukin 35 (IL35)

RPU54947-1mg 1mg
EUR 2730

Active Interleukin 35 (IL35)

RPU54947-50ug 50ug
EUR 495

Recombinant Interleukin 35 (IL35)

4-RPC008Hu01
  • EUR 618.89
  • EUR 289.20
  • EUR 1990.85
  • EUR 743.62
  • EUR 1367.23
  • EUR 489.60
  • EUR 4797.12
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 35 expressed in: E.coli

Recombinant Interleukin 35 (IL35)

4-RPC008Mu01
  • EUR 644.70
  • EUR 296.40
  • EUR 2087.62
  • EUR 775.87
  • EUR 1431.74
  • EUR 506.40
  • EUR 5039.04
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Interleukin 35 expressed in: E.coli

Recombinant Interleukin 35 (IL35)

RPU42301-100ug 100ug
EUR 570.9

Recombinant Interleukin 35 (IL35)

RPU42301-1mg 1mg
EUR 2533.7

Recombinant Interleukin 35 (IL35)

RPU42301-50ug 50ug
EUR 459.8

Recombinant Interleukin 35 (IL35)

RPU54474-100ug 100ug
EUR 601.7

Recombinant Interleukin 35 (IL35)

RPU54474-1mg 1mg
EUR 2665

Recombinant Interleukin 35 (IL35)

RPU54474-50ug 50ug
EUR 482.9

Interleukin 35 (IL35) Antibody Pair

20-abx370609
  • EUR 2097.60
  • EUR 1345.20
  • 10 × 96 tests
  • 5 × 96 tests

Interleukin 35 (IL35) Antibody (Biotin)

20-abx274523
  • EUR 427.20
  • EUR 243.60
  • EUR 1111.20
  • EUR 560.40
  • EUR 360.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 35 (IL35) Antibody (Biotin)

20-abx274658
  • EUR 543.60
  • EUR 292.80
  • EUR 1579.20
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Interleukin 35 (IL35) Monoclonal Antibody

CAU29857-100ul 100ul
EUR 248.6

Interleukin 35 (IL35) Monoclonal Antibody

CAU29857-200ul 200ul
EUR 310.8

Interleukin 35 (IL35) Polyclonal Antibody

CAU24862-100ul 100ul
EUR 241.9

Interleukin 35 (IL35) Polyclonal Antibody

CAU24862-200ul 200ul
EUR 302.4

Interleukin 35 (IL35) Polyclonal Antibody

CAU24863-100ul 100ul
EUR 235.2

Interleukin 35 (IL35) Polyclonal Antibody

CAU24863-200ul 200ul
EUR 294

Human Interleukin 35 (IL35) Protein

20-abx067529
  • EUR 861.60
  • EUR 343.20
  • EUR 2682.00
  • EUR 1028.40
  • EUR 610.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Interleukin 35 (IL35) Protein

20-abx167498
  • EUR 895.20
  • EUR 360.00
  • EUR 2815.20
  • EUR 1078.80
  • EUR 644.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

CLIA Kit for Interleukin 35 (IL35)

SCC008Mu 96Т
EUR 864

Interleukin 35 (IL35) Monoclonal Antibody (Human)

4-MAC008Hu21
  • EUR 306.00
  • EUR 3170.40
  • EUR 786.00
  • EUR 386.40
  • EUR 260.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35)

Interleukin 35 (IL35) Polyclonal Antibody (Human)

4-PAC008Hu01
  • EUR 296.40
  • EUR 3012.00
  • EUR 750.00
  • EUR 372.00
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35)

Interleukin 35 (IL35) Polyclonal Antibody (Mouse)

4-PAC008Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35)

Rat Interleukin 35 (IL35) ELISA Kit

abx363995-96tests 96 tests
EUR 943.2

ELISA Kit for Interleukin 35 (IL35)

SEC008Hu 96Т
EUR 700

Human Interleukin 35 (IL35) CLIA Kit

20-abx493363
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Interleukin 35 (IL35) CLIA Kit

EKN51079-48T 48T
EUR 426.72

Mouse Interleukin 35 (IL35) CLIA Kit

EKN51079-5x96T 5x96T
EUR 2895.6

Mouse Interleukin 35 (IL35) CLIA Kit

EKN51079-96T 96T
EUR 609.6

Mouse Interleukin 35 (IL35) CLIA Kit

EKU09968-48T 48T
EUR 685.72

Mouse Interleukin 35 (IL35) CLIA Kit

EKU09968-5x96T 5x96T
EUR 4653.1

Mouse Interleukin 35 (IL35) CLIA Kit

EKU09968-96T 96T
EUR 979.6

Mouse Interleukin 35 (IL35)CLIA Kit

SCC008Mu-10x96wellstestplate 10x96-wells test plate
EUR 6965.86
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35)CLIA Kit

SCC008Mu-1x48wellstestplate 1x48-wells test plate
EUR 678.84
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35)CLIA Kit

SCC008Mu-1x96wellstestplate 1x96-wells test plate
EUR 918.34
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35)CLIA Kit

SCC008Mu-5x96wellstestplate 5x96-wells test plate
EUR 3772.51
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) CLIA Kit

4-SCC008Mu
  • EUR 7026.00
  • EUR 3772.80
  • EUR 919.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Human Interleukin 35 (IL35) ELISA Kit

20-abx152044
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Interleukin 35 (IL35) ELISA Kit

abx254224-96tests 96 tests
EUR 801.6

Sheep Interleukin 35 (IL35) ELISA Kit

abx257113-96tests 96 tests
EUR 1036.8

Human Interleukin 35 (IL35) ELISA Kit

abx252675-96tests 96 tests
EUR 801.6

Mouse Interleukin 35 (IL35) ELISA Kit

20-abx154255
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Interleukin 35 (IL35) ELISA Kit

DLR-IL35-Hu-48T 48T
EUR 555.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 35 (IL35) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

DLR-IL35-Hu-96T 96T
EUR 718.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 35 (IL35) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

SEC008Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

SEC008Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

SEC008Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

SEC008Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

4-SEC008Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interleukin 35 (IL35) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Interleukin 35 (IL35) ELISA Kit

SEC008Mu-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

SEC008Mu-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

SEC008Mu-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

SEC008Mu-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

4-SEC008Mu
  • EUR 5895.60
  • EUR 3114.00
  • EUR 786.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Interleukin 35 (IL35) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Interleukin 35 (IL35) ELISA Kit

EKN51401-48T 48T
EUR 380.31

Human Interleukin 35 (IL35) ELISA Kit

EKN51401-5x96T 5x96T
EUR 2580.68

Human Interleukin 35 (IL35) ELISA Kit

EKN51401-96T 96T
EUR 543.3

Mouse Interleukin 35 (IL35) ELISA Kit

EKN52309-48T 48T
EUR 426.72

Mouse Interleukin 35 (IL35) ELISA Kit

EKN52309-5x96T 5x96T
EUR 2895.6

Mouse Interleukin 35 (IL35) ELISA Kit

EKN52309-96T 96T
EUR 609.6

Human Interleukin 35 (IL35) ELISA Kit

EKU05327-48T 48T
EUR 555.66

Human Interleukin 35 (IL35) ELISA Kit

EKU05327-5x96T 5x96T
EUR 3770.55

Human Interleukin 35 (IL35) ELISA Kit

EKU05327-96T 96T
EUR 793.8

Mouse Interleukin 35 (IL35) ELISA Kit

EKU05328-48T 48T
EUR 571.55

Mouse Interleukin 35 (IL35) ELISA Kit

EKU05328-5x96T 5x96T
EUR 3878.38

Mouse Interleukin 35 (IL35) ELISA Kit

EKU05328-96T 96T
EUR 816.5

Mouse Interleukin 35 (IL35) ELISA Kit

EKU11198-48T 48T
EUR 685.72

Mouse Interleukin 35 (IL35) ELISA Kit

EKU11198-5x96T 5x96T
EUR 4653.1

Mouse Interleukin 35 (IL35) ELISA Kit

EKU11198-96T 96T
EUR 979.6

Human Interleukin 35 (IL35) ELISA Kit

EKN46438-48T 48T
EUR 378.7

Human Interleukin 35 (IL35) ELISA Kit

EKN46438-5x96T 5x96T
EUR 2569.75

Human Interleukin 35 (IL35) ELISA Kit

EKN46438-96T 96T
EUR 541

Mouse Interleukin 35 (IL35) ELISA Kit

EKN46439-48T 48T
EUR 389.62

Mouse Interleukin 35 (IL35) ELISA Kit

EKN46439-5x96T 5x96T
EUR 2643.85

Mouse Interleukin 35 (IL35) ELISA Kit

EKN46439-96T 96T
EUR 556.6

Human Interleukin 35 (IL35) ELISA Kit

EKU10290-48T 48T
EUR 610.82

Human Interleukin 35 (IL35) ELISA Kit

EKU10290-5x96T 5x96T
EUR 4144.85

Human Interleukin 35 (IL35) ELISA Kit

EKU10290-96T 96T
EUR 872.6

Human Interleukin 35 (IL35) ELISA Kit

DL-IL35-Hu 96T
EUR 384
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

DLR-IL35-Hu 96T
EUR 404
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 35 (IL35) Protein (Active)

20-abx651393
  • EUR 910.80
  • EUR 360.00
  • EUR 2865.60
  • EUR 1095.60
  • EUR 644.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat IL35(Interleukin 35) ELISA Kit

ELK0632-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat IL35(Interleukin 35) ELISA Kit

ELK0632-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human IL35(Interleukin 35) ELISA Kit

ELK2745-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human IL35(Interleukin 35) ELISA Kit

ELK2745-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Interleukin 35 ELISA Kit (IL35)

RK01676 96 Tests
EUR 687.6

Mouse Interleukin 35 ELISA Kit (IL35)

RK02945 96 Tests
EUR 625.2

Mouse IL35(Interleukin 35) ELISA Kit

ELK5768-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse IL35(Interleukin 35) ELISA Kit

ELK5768-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Interleukin 35 (IL35) Monoclonal Antibody (Human), PE

4-MAC008Hu21-PE
  • EUR 367.20
  • EUR 3340.80
  • EUR 943.20
  • EUR 463.20
  • EUR 238.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with PE.

Interleukin 35 (IL35) Polyclonal Antibody (Human), PE

4-PAC008Hu01-PE
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with PE.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), PE

4-PAC008Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with PE.

Interleukin 35 (IL35) Monoclonal Antibody (Human), APC

4-MAC008Hu21-APC
  • EUR 429.60
  • EUR 4146.00
  • EUR 1148.40
  • EUR 549.60
  • EUR 268.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with APC.

Interleukin 35 (IL35) Monoclonal Antibody (Human), Cy3

4-MAC008Hu21-Cy3
  • EUR 522.00
  • EUR 5478.00
  • EUR 1482.00
  • EUR 682.80
  • EUR 309.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with Cy3.

Interleukin 35 (IL35) Monoclonal Antibody (Human), HRP

4-MAC008Hu21-HRP
  • EUR 392.40
  • EUR 3613.20
  • EUR 1015.20
  • EUR 495.60
  • EUR 253.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with HRP.

Interleukin 35 (IL35) Polyclonal Antibody (Human), APC

4-PAC008Hu01-APC
  • EUR 414.00
  • EUR 3930.00
  • EUR 1094.40
  • EUR 528.00
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with APC.

Interleukin 35 (IL35) Polyclonal Antibody (Human), Cy3

4-PAC008Hu01-Cy3
  • EUR 502.80
  • EUR 5190.00
  • EUR 1410.00
  • EUR 654.00
  • EUR 301.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with Cy3.

Interleukin 35 (IL35) Polyclonal Antibody (Human), HRP

4-PAC008Hu01-HRP
  • EUR 379.20
  • EUR 3426.00
  • EUR 968.40
  • EUR 477.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with HRP.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), APC

4-PAC008Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with APC.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), Cy3

4-PAC008Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with Cy3.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), HRP

4-PAC008Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with HRP.

Interleukin 35 (IL35) Monoclonal Antibody (Human), FITC

4-MAC008Hu21-FITC
  • EUR 367.20
  • EUR 3340.80
  • EUR 943.20
  • EUR 463.20
  • EUR 238.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with FITC.

Interleukin 35 (IL35) Polyclonal Antibody (Human), FITC

4-PAC008Hu01-FITC
  • EUR 355.20
  • EUR 3168.00
  • EUR 900.00
  • EUR 446.40
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with FITC.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), FITC

4-PAC008Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with FITC.

Interleukin 35 (IL35) Monoclonal Antibody (Human), Biotinylated

4-MAC008Hu21-Biotin
  • EUR 384.00
  • EUR 3110.40
  • EUR 912.00
  • EUR 472.80
  • EUR 267.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with Biotin.

Interleukin 35 (IL35) Polyclonal Antibody (Human), Biotinylated

4-PAC008Hu01-Biotin
  • EUR 373.20
  • EUR 2952.00
  • EUR 872.40
  • EUR 457.20
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with Biotin.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), Biotinylated

4-PAC008Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with Biotin.

Interleukin 35 (IL35) Magnetic Luminex Assay Kit

LKU605063-96T 96T
EUR 993.6

Interleukin 35 (IL35) Magnetic Luminex Assay Kit

LKU605066-96T 96T
EUR 966

ELISA kit for Human IL35 (Interleukin 35)

ELK2745 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Interleukin 35 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse IL35 (Interleukin 35)

ELK5768 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Interleukin 35 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Interleukin 35 (IL35) Monoclonal Antibody (Human), APC-Cy7

4-MAC008Hu21-APC-Cy7
  • EUR 715.20
  • EUR 8148.00
  • EUR 2154.00
  • EUR 955.20
  • EUR 394.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with APC-Cy7.

Interleukin 35 (IL35) Polyclonal Antibody (Human), APC-Cy7

4-PAC008Hu01-APC-Cy7
  • EUR 685.20
  • EUR 7716.00
  • EUR 2046.00
  • EUR 912.00
  • EUR 382.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with APC-Cy7.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), APC-Cy7

4-PAC008Mu01-APC-Cy7
  • EUR 699.60
  • EUR 7932.00
  • EUR 2100.00
  • EUR 933.60
  • EUR 388.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with APC-Cy7.

Mini Samples ELISA Kit for Interleukin 35 (IL35)

MEC008Mu 96Т
EUR 864

Mini Samples Mouse Interleukin 35 (IL35) ELISA Kit

MEC008Mu-10x96wellstestplate 10x96-wells test plate
EUR 6965.86
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 35 (IL35) ELISA Kit

MEC008Mu-1x48wellstestplate 1x48-wells test plate
EUR 678.84
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 35 (IL35) ELISA Kit

MEC008Mu-1x96wellstestplate 1x96-wells test plate
EUR 918.34
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 35 (IL35) ELISA Kit

MEC008Mu-5x96wellstestplate 5x96-wells test plate
EUR 3772.51
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mini Samples Mouse Interleukin 35 (IL35) ELISA Kit

4-MEC008Mu
  • EUR 7026.00
  • EUR 3712.80
  • EUR 919.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Interleukin 35 (IL35) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

High Sensitive ELISA Kit for Interleukin 35 (IL35)

HEC008Hu 96Т
EUR 770

Human High Sensitive Interleukin 35 (IL35) CLIA Kit

20-abx490722
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human High Sensitive Interleukin 35 (IL35) ELISA Kit

20-abx258386
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

High Sensitive Human Interleukin 35 (IL35) ELISA Kit

HEC008Hu-10x96wellstestplate 10x96-wells test plate
EUR 6227.58
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

High Sensitive Human Interleukin 35 (IL35) ELISA Kit

HEC008Hu-1x48wellstestplate 1x48-wells test plate
EUR 618.04
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

High Sensitive Human Interleukin 35 (IL35) ELISA Kit

HEC008Hu-1x96wellstestplate 1x96-wells test plate
EUR 831.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

In addition, the LN suppressed mechanical allodynia in a rat model of OA PMM. The results support the concept that administration of LN can provide potential therapy in OA.