Bromodomain-containing protein 4 inhibition alleviates matrix degradation by enhancing autophagy and suppressing NLRP3 inflammasome activity in NP cells.

Imbalance between matrix synthesis and degradation is the hallmark of the degeneration of the intervertebral disc while contributing inflammatory cytokine imbalance. Bromodomain and extra-terminal domain (BET) family associated with the pathogenesis of inflammation, and inhibition of BRD4, an important member of the family BET, play an anti-inflammatory role in many diseases.

However, it remains elusive whether BRD4 play the same role in the nucleus pulposus (NP) cells and participates in the pathogenesis of intervertebral disc degeneration. The purpose of this study to examine whether inhibition of BRD4 matrix regulate metabolism by controlling autophagy and NLRP3 inflammasome activity. In addition, it investigated the relationship between nuclear factor kB (NF-kB) signaling, autophagy and NLRP3 inflammasome in NP cells.

Here, real-time polymerase chain reaction, Western blot analysis and GFP-LC3 adenoviral vector transduction in vitro were used, and it was revealed that BRD4 inhibition of matrix degradation and enhance ease of autophagy in the presence or absence of tumor necrosis factor α. In addition, p65 knockdown or treatment with JQ1 and Bay11-7082 indicate that inhibition of BRD4 NLRP3 inflammasome activity weakened through NF-kB signaling, while autophagy inhibition by bafilomycin A1 promoted matrix degradation and cell activity in the NP NLRP3 inflammasome.

In addition, the analysis of RNA expression in tissues BRD4 human NP further verified BRD4 destructive functions. Simply, BRD4 inhibition of matrix degradation Easing by increasing autophagy and suppress the activity of NLRP3 inflammasome through the NF-kB signaling in cells NP.

Bromodomain-containing protein 4 inhibition alleviates matrix degradation by enhancing autophagy and suppressing NLRP3 inflammasome activity in NP cells.
Bromodomain-containing protein 4 inhibition alleviates matrix degradation by enhancing autophagy and suppressing NLRP3 inflammasome activity in NP cells.

Links N Pressing interleukin-1β-induced biological effects in human osteoarthritic cartilage.

Osteoarthritis (OA) is a diarthrodial joint disease associated with proteolytic degradation of extracellular matrix under conditions of inflammation, pain and disability. Currently, no therapies to prevent, reverse or modulate the course of disease. This study aimed to evaluate the potential of regenerative Link N (LN) in human OA cartilage in inflammatory environment and determine whether the LN can influence the behavior associated pain in OA knee injury model rats. Osteo-chondro explants OA and OA chondrocytes treated with LN in the presence of interleukin-1β (IL-1β) to simulate the environment of osteoarthritic.

Von Frey quantitative polymerase chain reaction and Western blotting was performed to determine the effect of LN on matrix protein synthesis, catabolic enzymes, cytokines and nerve growth factor expression. Partial medial meniscectomy (PMM) was performed on the knee C57BL / 6 mice and, 12 weeks post-surgery, the rats were given 5 mg of intra-articular injection of LN or phosphate-buffered saline. A von Frey test carried out for 24 hours to measure the mechanical allodynia in the hind legs.

LN modulated proteoglycans and collagen synthesis in human OA cartilage by inhibiting the biological effects of IL-1β-induced. LN also suppressed the upregulation of IL-1β-induced cartilage-degrading enzymes and inflammatory molecules in OA chondrocytes. After an investigation of the canonical signaling pathway of IL-1β and nuclear factor kappa-light-chain-enhancer activated B cells (NF-kB), LN resulted in significantly inhibit NF-kB activation in a dose-dependent manner.

Interleukin 35 (IL35) Antibody

20-abx132303
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  • 1 mg
  • 200 ug

Interleukin 35 (IL35) Antibody

abx129830-100l 100 µl
EUR 275

Interleukin 35 (IL35) Antibody

abx129830-1ml 1 ml
EUR 775

Interleukin 35 (IL35) Antibody

abx129830-200l 200 µl
EUR 350

Interleukin 35 (IL35) Antibody

abx132303-100tests 100 tests
EUR 275

Interleukin 35 (IL35) Antibody

abx101210-100l 100 µl
EUR 275

Interleukin 35 (IL35) Antibody

abx101210-1ml 1 ml
EUR 750

Interleukin 35 (IL35) Antibody

abx101210-200l 200 µl
EUR 337.5

Active Interleukin 35 (IL35)

APC008Hu01 10ug
EUR 200

Active Interleukin 35 (IL35)

4-APC008Hu01
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  • 5 mg
Description: Recombinant Human Interleukin 35 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 35 (IL35)

RPU54947-100ug 100ug
EUR 616

Active Interleukin 35 (IL35)

RPU54947-1mg 1mg
EUR 2730

Active Interleukin 35 (IL35)

RPU54947-50ug 50ug
EUR 495

Recombinant Interleukin 35 (IL35)

RPU42301-100ug 100ug
EUR 570.9

Recombinant Interleukin 35 (IL35)

RPU42301-1mg 1mg
EUR 2533.7

Recombinant Interleukin 35 (IL35)

RPU42301-50ug 50ug
EUR 459.8

Recombinant Interleukin 35 (IL35)

RPU54474-100ug 100ug
EUR 601.7

Recombinant Interleukin 35 (IL35)

RPU54474-1mg 1mg
EUR 2665

Recombinant Interleukin 35 (IL35)

RPU54474-50ug 50ug
EUR 482.9

Recombinant Interleukin 35 (IL35)

RPC008Hu01 10ug
EUR 186

Recombinant Interleukin 35 (IL35)

4-RPC008Hu01
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  • 100 ug
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Description: Recombinant Human Interleukin 35 expressed in: E.coli

Recombinant Interleukin 35 (IL35)

RPC008Mu01 10ug
EUR 195

Recombinant Interleukin 35 (IL35)

4-RPC008Mu01
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Description: Recombinant Mouse Interleukin 35 expressed in: E.coli

Interleukin 35 (IL35) Antibody Pair

20-abx370609
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  • 10 × 96 tests
  • 5 × 96 tests

Interleukin 35 (IL35) Antibody Pair

abx370609-100g 100 µg
EUR 2150

Interleukin 35 (IL35) Antibody Pair

abx370609-50g 50 µg
EUR 1337.5

Interleukin 35 (IL35) Antibody (Biotin)

20-abx274523
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  • 100 ug
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Interleukin 35 (IL35) Antibody (Biotin)

20-abx274658
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  • 100 ug
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Interleukin 35 (IL35) Antibody (Biotin)

abx274658-100tests 100 tests
EUR 362.5

Interleukin 35 (IL35) Monoclonal Antibody

CAU29857-100ul 100ul
EUR 248.6

Interleukin 35 (IL35) Monoclonal Antibody

CAU29857-200ul 200ul
EUR 310.8

Interleukin 35 (IL35) Polyclonal Antibody

CAU24862-100ul 100ul
EUR 241.9

Interleukin 35 (IL35) Polyclonal Antibody

CAU24862-200ul 200ul
EUR 302.4

Interleukin 35 (IL35) Polyclonal Antibody

CAU24863-100ul 100ul
EUR 235.2

Interleukin 35 (IL35) Polyclonal Antibody

CAU24863-200ul 200ul
EUR 294

Human Interleukin 35 (IL35) Protein

20-abx067529
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  • 100 ug
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Mouse Interleukin 35 (IL35) Protein

20-abx167498
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  • 100 ug
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Human Interleukin 35 (IL35) Protein

abx067529-100g 100 µg
EUR 600

Human Interleukin 35 (IL35) Protein

abx067529-10g 10 µg
EUR 225

Human Interleukin 35 (IL35) Protein

abx067529-50g 50 µg
EUR 412.5

Mouse Interleukin 35 (IL35) Protein

abx167498-1ml 1 ml
EUR 237.5

Human Interleukin 35 (IL35) Protein

abx682058-100g 100 µg
EUR 837.5

Human Interleukin 35 (IL35) Protein

abx682058-20g 20 µg
EUR 325

Monoclonal Antibody to Interleukin 35 (IL35)

MAC008Hu21 100ul
EUR 259

Polyclonal Antibody to Interleukin 35 (IL35)

PAC008Hu01 100ul
EUR 245

Polyclonal Antibody to Interleukin 35 (IL35)

PAC008Mu01 100ul
EUR 252

CLIA Kit for Interleukin 35 (IL35)

SCC008Mu 96Т
EUR 864

Rat Interleukin 35 (IL35) ELISA Kit

abx363995-96tests 96 tests
EUR 943.2

ELISA Kit for Interleukin 35 (IL35)

SEC008Hu 96Т
EUR 700

Interleukin 35 (IL35) Monoclonal Antibody (Human)

4-MAC008Hu21
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  • 100ul
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Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35)

Interleukin 35 (IL35) Polyclonal Antibody (Human)

4-PAC008Hu01
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35)

Interleukin 35 (IL35) Polyclonal Antibody (Mouse)

4-PAC008Mu01
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  • 100ul
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Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35)

Mouse Interleukin 35 (IL35) CLIA Kit

EKU09968-48T 48T
EUR 685.72

Mouse Interleukin 35 (IL35) CLIA Kit

EKU09968-5x96T 5x96T
EUR 4653.1

Mouse Interleukin 35 (IL35) CLIA Kit

EKU09968-96T 96T
EUR 979.6

Mouse Interleukin 35 (IL35) CLIA Kit

EKN51079-48T 48T
EUR 426.72

Mouse Interleukin 35 (IL35) CLIA Kit

EKN51079-5x96T 5x96T
EUR 2895.6

Mouse Interleukin 35 (IL35) CLIA Kit

EKN51079-96T 96T
EUR 609.6

Human Interleukin 35 (IL35) CLIA Kit

20-abx493363
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Interleukin 35 (IL35)CLIA Kit

SCC008Mu-10x96wellstestplate 10x96-wells test plate
EUR 6965.86
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35)CLIA Kit

SCC008Mu-1x48wellstestplate 1x48-wells test plate
EUR 678.84
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35)CLIA Kit

SCC008Mu-1x96wellstestplate 1x96-wells test plate
EUR 918.34
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35)CLIA Kit

SCC008Mu-5x96wellstestplate 5x96-wells test plate
EUR 3772.51
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) CLIA Kit

4-SCC008Mu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Mouse Interleukin 35 (IL35)Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Human Interleukin 35 (IL35) CLIA Kit

abx197194-100l 100 µl Ask for price

Human Interleukin 35 (IL35) CLIA Kit

abx197194-50l 50 µl
EUR 618.75

Mouse Interleukin 35 (IL35) CLIA Kit

abx197195-100l 100 µl Ask for price

Mouse Interleukin 35 (IL35) CLIA Kit

abx197195-50l 50 µl
EUR 618.75

Human Interleukin 35 (IL35) ELISA Kit

DLR-IL35-Hu 96T
EUR 404
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

DLR-IL35-Hu-48T 48T
EUR 555.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 35 (IL35) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

DLR-IL35-Hu-96T 96T
EUR 718.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 35 (IL35) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

EKU11198-48T 48T
EUR 685.72

Mouse Interleukin 35 (IL35) ELISA Kit

EKU11198-5x96T 5x96T
EUR 4653.1

Mouse Interleukin 35 (IL35) ELISA Kit

EKU11198-96T 96T
EUR 979.6

Human Interleukin 35 (IL35) ELISA Kit

EKU10290-48T 48T
EUR 610.82

Human Interleukin 35 (IL35) ELISA Kit

EKU10290-5x96T 5x96T
EUR 4144.85

Human Interleukin 35 (IL35) ELISA Kit

EKU10290-96T 96T
EUR 872.6

Human Interleukin 35 (IL35) ELISA Kit

EKN46438-48T 48T
EUR 378.7

Human Interleukin 35 (IL35) ELISA Kit

EKN46438-5x96T 5x96T
EUR 2569.75

Human Interleukin 35 (IL35) ELISA Kit

EKN46438-96T 96T
EUR 541

Mouse Interleukin 35 (IL35) ELISA Kit

EKN46439-48T 48T
EUR 389.62

Mouse Interleukin 35 (IL35) ELISA Kit

EKN46439-5x96T 5x96T
EUR 2643.85

Mouse Interleukin 35 (IL35) ELISA Kit

EKN46439-96T 96T
EUR 556.6

Human Interleukin 35 (IL35) ELISA Kit

EKN51401-48T 48T
EUR 380.31

Human Interleukin 35 (IL35) ELISA Kit

EKN51401-5x96T 5x96T
EUR 2580.68

Human Interleukin 35 (IL35) ELISA Kit

EKN51401-96T 96T
EUR 543.3

Mouse Interleukin 35 (IL35) ELISA Kit

EKN52309-48T 48T
EUR 426.72

Mouse Interleukin 35 (IL35) ELISA Kit

EKN52309-5x96T 5x96T
EUR 2895.6

Mouse Interleukin 35 (IL35) ELISA Kit

EKN52309-96T 96T
EUR 609.6

Human Interleukin 35 (IL35) ELISA Kit

EKU05327-48T 48T
EUR 555.66

Human Interleukin 35 (IL35) ELISA Kit

EKU05327-5x96T 5x96T
EUR 3770.55

Human Interleukin 35 (IL35) ELISA Kit

EKU05327-96T 96T
EUR 793.8

Mouse Interleukin 35 (IL35) ELISA Kit

EKU05328-48T 48T
EUR 571.55

Mouse Interleukin 35 (IL35) ELISA Kit

EKU05328-5x96T 5x96T
EUR 3878.38

Mouse Interleukin 35 (IL35) ELISA Kit

EKU05328-96T 96T
EUR 816.5

Human Interleukin 35 (IL35) ELISA Kit

DL-IL35-Hu 96T
EUR 384
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Sheep Interleukin 35 (IL35) ELISA Kit

abx257113-96tests 96 tests
EUR 1036.8

Human Interleukin 35 (IL35) ELISA Kit

abx252675-96tests 96 tests
EUR 801.6

Mouse Interleukin 35 (IL35) ELISA Kit

abx254224-96tests 96 tests
EUR 801.6

Human Interleukin 35 (IL35) ELISA Kit

20-abx152044
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Interleukin 35 (IL35) ELISA Kit

20-abx154255
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Interleukin 35 (IL35) ELISA Kit

SEC008Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

SEC008Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

SEC008Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

SEC008Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Interleukin 35 (IL35) ELISA Kit

4-SEC008Hu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interleukin 35 (IL35) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Interleukin 35 (IL35) ELISA Kit

SEC008Mu-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

SEC008Mu-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

SEC008Mu-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

SEC008Mu-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 35 (IL35) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Interleukin 35 (IL35) ELISA Kit

4-SEC008Mu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Interleukin 35 (IL35) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Interleukin 35 (IL35) Protein (Active)

20-abx651393
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  • 100 ug
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  • 1 mg
  • 200 ug
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Human Interleukin 35 (IL35) Protein (Active)

abx651393-5mg 5 mg
EUR 637.5

Rat IL35(Interleukin 35) ELISA Kit

ELK0632-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat IL35(Interleukin 35) ELISA Kit

ELK0632-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse IL35(Interleukin 35) ELISA Kit

ELK5768-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse IL35(Interleukin 35) ELISA Kit

ELK5768-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human IL35(Interleukin 35) ELISA Kit

ELK2745-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human IL35(Interleukin 35) ELISA Kit

ELK2745-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL35 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Interleukin 35 ELISA Kit (IL35)

RK01676 96 Tests
EUR 687.6

Mouse Interleukin 35 ELISA Kit (IL35)

RK02945 96 Tests
EUR 625.2

Interleukin 35 (IL35) Monoclonal Antibody (Human), PE

4-MAC008Hu21-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with PE.

Interleukin 35 (IL35) Polyclonal Antibody (Human), PE

4-PAC008Hu01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with PE.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), PE

4-PAC008Mu01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with PE.

Interleukin 35 (IL35) Monoclonal Antibody (Human), APC

4-MAC008Hu21-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with APC.

Interleukin 35 (IL35) Monoclonal Antibody (Human), Cy3

4-MAC008Hu21-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with Cy3.

Interleukin 35 (IL35) Monoclonal Antibody (Human), HRP

4-MAC008Hu21-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with HRP.

Interleukin 35 (IL35) Polyclonal Antibody (Human), APC

4-PAC008Hu01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with APC.

Interleukin 35 (IL35) Polyclonal Antibody (Human), Cy3

4-PAC008Hu01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with Cy3.

Interleukin 35 (IL35) Polyclonal Antibody (Human), HRP

4-PAC008Hu01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Interleukin 35 (IL35). This antibody is labeled with HRP.

Interleukin 35 (IL35) Polyclonal Antibody (Mouse), APC

4-PAC008Mu01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Interleukin 35 (IL35). This antibody is labeled with APC.

In addition, the LN suppressed mechanical allodynia in a rat model of OA PMM. The results support the concept that administration of LN can provide potential therapy in OA.